Volume: 54 Issue: 4
Year: 2023, Page: 916-920, Doi: https://doi.org/10.51966/jvas.2023.54.4.916-920
Received: Dec. 17, 2022 Accepted: Dec. 21, 2022 Published: Dec. 31, 2023
Extra cellular matrix (ECM) incorporated calcium hydroxyapatite bound with triphasic silica is a custom made bone graft with hybrid properties which is used for bone regeneration. A promising new technique for preparing natural matrices for bone regeneration now in trend is decellularisation. According to reports, the ECM activates mesenchymal stem cells, speeding up bone healing. Although research employing hard tissues like bone are scarce, successful decellularisation has been done using a variety of tissues including skin, cartilage and tendon. The study was carried out in rat calvaria; critical size defects were created and later grafted with decellularised tissue engineered HASi bioceramic (test) and plain HASi bioceramic (control) on left and right side respectively. Radiography was used to assess changes in the graft, the rate of its degradation and the degree of bone repair. Postoperative dorsoventral view of the skull was taken immediately after surgery and thereafter on 2nd, 4th, 8th and 12th week post-surgery. The grafts were found to remain stable throughout the healing period. By 4th week onwards signs of gradual degradation of material with regeneration of bone could be noticed especially on test side compared to control. Test material
Keywords:
Bisht, B., Hope, A., Mukherjee, A. and Paul, M. K. 2021. Advances in the fabrication of scaffold and 3D printing of biomimetic bone graft. Annals of Biomed. Engineering, 49:1128-1150.
Dinesh, P.T., Syam K. Venugopal, John Martin K. D., Devanand C. B., Usha N. Pillai and N.D Nair. 2018. Radiographic evaluation of healing of critical size defects of femur treated with tri-phasic composite bio-ceramic implants in rat models. Ind.J.Nat.Sci. 9:15504-15507.
Einhorn, T. A. and L. C. Gerstenfeld. 2015. Fracture healing: mechanisms and interventions. Nat. Rev. Rheumatol.11:45–54.
Nair M. B. 2009. Bone reconstruction of goat femur segmental defects using tissue engineered bioceramic scaffolds with osteogenically induced mesenchymal stem cells and platelet rich plasma. Ph.D. Thesis, Sri ChithiraThirunal Institute for Medical Science and Technology, Trivandrum, 235p
Rao, R., Dinesh, P.T., Sooryadas, S., Chandy, G. and Mathew, M. 2021. Successful utilization of triphasic silica containing ceramic coated hydroxyapatite (HASi) for the treatment of comminuted tibial fracture in a goat: a case report. J. Vet. Anim. Sci. 52: 200-203
Spicer, P.P., Kretlow, J.D., Young, S., Jansen, J.A., Kasper, F.K. and Mikos, A.G. 2012. Evaluation of bone regeneration using the rat critical size calvarial defect. Nature protocols. 7:1918-1929. Suresh, D., Syam, K.V., Chandy, G., Sooryadas, S. and Deepa, P.M. 2017. Radiographical evaluation of Sr-HASi bioceramic substitute for critical size bone defect in a goat–Clinical study. J. Vet. Anim.
Sci. 48: pp.40-42.
© 2023 Manasa et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted
Manasa M., Dinesh P.T., Fernandez F.B., Sooryadas S., Palekkodan H., Anoop S., Jineshkumar N.S., Remya V. and Varma H.K. 2023. Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HASi in rat models. J. Vet. Anim. Sci. 54(4):916-920