Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 4

  • Open Access
  • Research Article

Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HAS i in rat models#

M. Manasa1, P.T. Dinesh*2, F.B. Fernandez3, S. Sooryadas2, Hamza Palekkodan4, S. Anoop5, N. S. Jineshkumar2, V. Remya2 and H.K. Varma6

# Part of MVSc thesis submitted to Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala

1 MVSc Scholar
2 Assistant professor,
3 Scientist C, Division of Bioceramics, BMT wing, SCTIMST Thiruvananthapuram
4 Assistant professor, Department of Veterinary Pathology
5 Professor and head,
6 Head BMT wing, SCTIMST Thiruvananthapuram

*Corresponding author: [email protected] Ph. +91 9447144085

Year: 2023, Page: 916-920, Doi:

Received: Dec. 17, 2022 Accepted: Dec. 21, 2022 Published: Dec. 31, 2023


Extra cellular matrix (ECM) incorporated calcium hydroxyapatite bound with triphasic silica is a custom made bone graft with hybrid properties which is used for bone regeneration. A promising new technique for preparing natural matrices for bone regeneration now in trend is decellularisation. According to reports, the ECM activates mesenchymal stem cells, speeding up bone healing. Although research employing hard tissues like bone are scarce, successful decellularisation has been done using a variety of tissues including skin, cartilage and tendon. The study was carried out in rat calvaria; critical size defects were created and later grafted with decellularised tissue engineered HASi bioceramic (test) and plain HASi bioceramic (control) on left and right side respectively. Radiography was used to assess changes in the graft, the rate of its degradation and the degree of bone repair. Postoperative dorsoventral view of the skull was taken immediately after surgery and thereafter on 2nd, 4th, 8th and 12th week post-surgery. The grafts were found to remain stable throughout the healing period. By 4th week onwards signs of gradual degradation of material with regeneration of bone could be noticed especially on test side compared to control. Test material



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Cite this article

Manasa M., Dinesh P.T., Fernandez F.B., Sooryadas S., Palekkodan H., Anoop S., Jineshkumar N.S., Remya V. and Varma H.K. 2023. Radiographic evaluation of healing of critical size calvarial defects treated with decellularised tissue engineered HASi in rat models. J. Vet. Anim. Sci. 54(4):916-920