Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 4

  • Open Access
  • Research Article

Semen quality characteristics of Aseel, Kadaknath and native chicken of Kerala#

D. Akshay1*, S. Harikrishnan2, Hiron M. Harshan3, P. Anitha4 and P.G. Vineetha5

#Part of MVSc thesis submitted to Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala

1 MVSc. Scholar, Department of Poultry Science, CVAS, Mannuthy
Assistant Professor and special officer, CASM Thiruvazhamkunnu
3 Associate professor, Department of Animal reproduction Gynaecology and Obstetrics
Professor and Head, Department of Poultry Science, CVAS, Mannuthy
5 Assistant Professor, CASM Thiruvazhamkunnu

*Corresponding author: [email protected], Ph. 9656578057

Year: 2023, Page: 882-890, Doi:

Received: July 11, 2022 Accepted: Nov. 1, 2023 Published: Dec. 31, 2023


The present study was conducted to compare the microscopic and macroscopic parameters of semen of three Indian indigenous chicken breeds viz. Aseel, Kadaknath and native chicken of Kerala. The semen volume in native chicken of Kerala was significantly higher compared to that of Aseel and Kadaknath roosters. Significantly (p<0.05) higher appearance score was observed in native chicken of Kerala and Kadaknath roosters compared to that of Aseel roosters. The pH of semen was found slightly alkaline in all the three breeds. Most of the semen samples were white in colour. Most of the semen samples were thick in Kadaknath roosters, thick and medium thick in native chicken of Kerala and thick, medium thick and watery in Aseel roosters. The major contaminants of semen observed were faeces, blood and uric acid crystals. Significantly (p<0.01) higher mass activity score and initial progressive motility was measured in native chicken of Kerala compared to Aseel roosters and the values of Kadaknath roosters were comparable to both. There was no significant difference in sperm concentration and sperm abnormality among three indigenous breeds. The sperm viability percentage was significantly (p<0.05) higher in native chicken of Kerala compared to Aseel and Kadaknath roosters. The characteristics of fresh semen samples collected from all the three breeds revealed that all three were fit for artificial insemination.

Keywords: Semen, Aseel, Kadaknath, native chicken, Kerala, artificial insemination


Abaza, M., Elnagar, A.S. and Azza, El-Sebai. 2003. Thyroid effects on semen quality and immune responses of mature Alexandria cockerels. Egyptian Poult. Sci. 23: 721-736. 

Al-Daraji, H.J., Al-Rawi, A.J. and Al-Tikriti, B.T.O. 2002. Study of the semen traits of Barred Plymouth rock, New Hampshire and local roosters. Iraqi J. Agric. Sci. 33: 255-260.

Bah, A.S., Chandhari, S.U.R. and Al-Amin, J.D. 2001. Semen characteristics of local breeder cocks in the sahel region of Nigeria. Rev. élev. méd. vét. pays trop. 54(2): 153-158.

BIS [Bureau of Indian Standards]. 2007. Poultry Feed Specification. (5th Ed.). Bureau of Indian Standards, New Delhi, 30p.

Biswas, A., Mohan, J. and Sastry, K.V.H. 2009. Effect of higher dietary vitamin E concentrations on physical and biochemical characteristics of semen in Kadaknath cockerels. Br. Poult. Sci. 50: 733-738.

Chauhan, P.M., Suthar, B.N., Nakhashi, H.C. and Sharma, V.K. 2017. Response of Gramapriya hybrid cock spermatozoa to hypo-osmotic swelling test and its correlation with seminal attributes. Indian. J. Poult. Sci. 52(1): 120-123.

Churchil, R.R., John, L., Praveena, P.E. and Cyriac, S. 2019. Strain and age-related changes of semen attributes in white leghorn roosters. Int. J. Chem. Stud. 7: 1838-1842.

Donoghue, A.M., Holsberger, D.R., Evenson, D.P. and Froman, D.P. 1998. Semen donor selection by in vitro sperm mobility increases fertility and semen storage in the turkey hen. J. Andrology, 19: 295- 301.

Etches, R.J. 1996. Reproduction in Poultry. (1st Ed.). CAB International, Wallingford, Oxon, UK, 318p. Getachew, T. 2016. A review article of artificial insemination in poultry. World Vet. J. 6(1): 42-51.

Gordon, I. 2005. Reproductive Technologies in Farm Animals. (2nd Ed.). CABI Publishing, UK, pp. 16-28.

Hafez, E.S.E. and Hafez, B. 2013. Reproduction in Farm Animals. (7th Ed.). Lippincott Williams & Wilkins, USA, 237p.

Haunshi, S., Doley, S. and Kadirvel, G. 2010. Comparative studies on egg, meat and semen qualities of native and improved chicken varieties developed for backyard poultry production. Trop. Anim. Hlth. Prod. 42: 1013-1019.

Haunshi, S., Niranjan, M., Shanmugam, M., Padhi, M.K., Reddy, M.R., Sunitha, R., Rajkumar, U. and Panda, A.K. 2011. Characterization of two Indian native chicken breeds for production, egg and semen quality and welfare traits. Poult. Sci. 90: 314-320. 

Inyawilert, W., Rungruangsak, J., Chanthi, S., Liao, Y.J., Phinyo, M., Tang, P.C. and Nfor, O.N. 2019. Age-related difference changes semen quality and seminal plasma protein patterns of Thai native rooster. Int. J. Agric. Technol. 15: 287- 296.

Jabbar, A., Abbass, W., Riaz, A., Ahmad, M., Sattar, A. and Akram, M. 2015. Effects of induced moulting on semen quality of indigenous chicken Aseel. Pak. J. Zool. 47(4): 1199-1200.

Lake, P.E. and Stewart, J.M. 1978. Artificial Insemination in Poultry. (1st Ed.). Her Majesty’s Stationery Office, UK, 33p. Lake, P.E. 1985. Fowl semen as collected by the massage method. J. Agric. Sci. 49(1): 120-126.

Latif, A., Ijaz, A., Aleem, M. and Mahmud, A. 2005. Effect of osmotic pressure and pH on the short-term storage and fertility of broiler breeder sperm. Pak. Vet. J. 25: 179.

Mavi, G.K., Dubey, P.P. and Cheema, R.S. 2017. Comparison of sperm attributes in two indigenous layer breeds and their relationship with fertility. Theriogenology Insight-An Int. J. Reprod. Anim. 7(2): 123-129.

McDaniel, G.R. and Craig, J.V. 1959. Behaviour traits, semen measurements and fertility of white leghorn males. Poult. Sci. 38: 1005-1014.

Mohan, J., Singh, R.P., Sastry, K.V.H., Moudgal, R.P., Biswas, A. and Shit, N. 2011. Influence of chicken native breeds on some physical and biochemical characteristics and short-term storage of semen. Br. Poult. Sci. 52(3): 395-400.

Mussa, N.J., Ratchamak, R., Ratsiri, T., Vongpralub, T., Boonkum, W., Semaming, Y. and Chankitisakul, V. 2021. Lipid profile of sperm cells in Thai native and commercial roosters and its impact on cryopreserved semen quality. Trop. Anim. Hlth. Prod. 53: 1-9.

Peters, S.O., Shoyebo, O.D., Ilori, B.M., Ozoje, M.O., Ikeobi, C.O.N. and Adebambo, O.A. 2008. Semen quality traits of seven strains of chickens raised in the humid tropics. Int. J. Poult. Sci. 7(10): 949-953.

Raghavendra, A., Simon, S., Jayakumar, C., Hiron, M.H., and Syam, K.V. 2022. Alkaline phosphatase in seminal plasma of sperm-rich fraction of semen in fertile and subfertile dogs. J. Vet. Anim. Sci. 53(2): 148-153.

Rakha, B.A., Hussain, I., Asma-ul-Husna, Malik, M.F., Akhter, S. and Ansari, M.S. 2015. Impact of ejaculate frequencies on the quality of Red Jungle Fowl (Gallus gallus murghi) semen. Avian. Biol. Res. 8(2): 109-112.

Ramamurthy, N., Narahari, D., Kothandaraman, P. and Sundararasu, V. 1986. Studies in the semen characteristics and their association with age and body weight of White Cornish sires. Indian. J. Poult. Sci. 21: 291-295.

Ramamurthy, N., Narahari, D., Kumararaj, R and Alfred-Jayaprasad, I. 1998. Semen characteristics of different breeds of chicken. J. Vet. Anim. Sci. 19: 15-18.

Santiago-Moreno, J., Castano, C., Coloma, M.A., Gomez-Brunet, A., Toledano-Diaz, A., Lopez-Sebastian, A. and Campo, J.L. 2009. Use of the hypo-osmotic swelling test and aniline blue staining to improve the evaluation of seasonal sperm variation in native Spanish free-range poultry. Poult. Sci. 88: 2661–2669.

Shukla, M.K., 2011. Applied veterinary andrology and frozen semen technology. New India Publishing Agency, 532p. Siudzinska, A. and Lukaszewicz, E. 2008. Effect of semen extenders and storage time on sperm morphology of four chicken breeds. J. Appl. Poult. Res. 17(1):101- 108.

Stella, C. 2011. Semen quality characteristics of Kuttanad, White Pekin (Anas platyrhynchos domesticus) and Muscovy (Cairina moschata momelanotus) ducks. M.V.Sc. thesis, Kerala Veterinary and Animal Sciences University, Pookode, 122p.

Sun, L., He, M., Wu, C., Zhang, S., Dai, J. and Zhang, D. 2021. Beneficial influence of soybean lecithin nanoparticles on rooster frozen–thawed semen quality and fertility. Animals, 11(6): 1769.

Sutiyono, S., Kurnianto, E., Sutopo, S., Ariyanto, D.K. and Samsudewa, D. 2021. Evaluation of native rooster based on breeding value of trait of macroscopic and microscopic semen quality. J. Sain Peternakan Indonesia, 16(3): 228-232.

Tabatabaei, S., Batavani, R.A. and Talebi, A.R. 2009. Comparison of semen quality in indigenous and Ross broiler breeder roosters. J. Anim. Vet. Adv. 8(1): 90-93.

Tabatabaei, S., Chaji, M. and Mohammadabadi, T. 2010. Correlation between age of rooster and semen quality in Iranian indigenous broiler breeder chickens. J. Anim. Vet. Adv. 9(1): 195-198.

Tarif, A.M., Bhuiyan, M.M.U., Ferdousy, R.N., Juyena, N.S. and Mollah, M.B.R. 2013. Evaluation of semen quality among four chicken lines. IOSR J. Agric. Vet. Sci. 6(5): 7-13.

Tuncer, P.B., Kinet, H. and Ozdogan, N. 2006. Evaluation of some spermatological characteristics in Denizli cocks. Vet. Fak. Derg. 3(1): 37-42. Wheeler, N.C. and Andrews, F.N. 1943. The influence of season on semen production in the domestic fowl. Poult. Sci. 22: 361- 367.

Yousaf, A., Rubab, F., Shahnawaz, R., Jamil, T., Iqbal, T., Bibi, N. and Haider, I. 2016. Impact of semen quality of Aseel chicken on induced moulting. J. Anim. Feed Res. 6(6): 130-132.

Cite this article

Akshay, D., Harikrishnan, S., Harshan, H.H., Anitha, P. and Vineetha, P.G. 2023. Semen quality characteristics of Aseel, Kadaknath and native chicken of Kerala. J. Vet. Anim. Sci. 54(4):882-890.