Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 2

  • Open Access
  • Research Article

Ameliorative effects of Bronco-T on formaldehyde-induced biochemical disruptions in the liver and muscle of male albino rats

B. Sujatha1, Payani Sholapuri1 and Matcha Bhaskar1*

1. Department of Zoology Sri Venkateswara University, Tirupati – 517502 India

*Corresponding author:[email protected], Ph. +91 9959911927

Year: 2024, Page: 291-303, Doi: https://doi.org/10.51966/jvas.2024.55.2.291-303

Received: Aug. 28, 2023 Accepted: Jan. 29, 2024 Published: June 30, 2024

Abstract

The objective of this investigation was to assess the potential protective effect of the herbal formulation Bronco-T against deleterious biochemical perturbations induced by formaldehyde in the liver and muscle tissues of male albino rats. The study aims to elucidate how formaldehyde exposure influences the equilibrium of protein, carbohydrate, antioxidants, and oxidative processes within the liver and muscle tissues of albino rats.Additionally, we investigated the direct relationship between observed biochemical alterations and the degree of formaldehyde exposure. In response to formaldehyde-induced abnormalities, administration of Bronco-T demonstrates significant promise.Bronco-T effectively mitigated aberrations in oxidative and antioxidant enzymes, restoring a more balanced state.Furthermore, Bronco-T facilitated improvements in protein and carbohydrate metabolism, playing a pivotal role in addressing the cascade of biochemical changes induced by formaldehyde exposure. It is noteworthy that inhALTion-based exposure to formaldehyde was associated with notable modifications in muscle and liver tissues. Through a comprehensive exploration of these dynamics, our study provides valuable insights into the potential protective role of Bronco-T against formaldehyde-induced biochemical disruptions in liver and muscle tissues, shedding light on its broader implications for health and well-being.

Keywords: Formaldehyde, toxicity, metabolism, Bronco-T, protective effects

References

Abdel Hamid, W.M., ElMoslemany, A.M. and Zeima, N.M. 2022. The potential protective effects of aqueous extracts of some herbs on renal toxicity induced by formaldehyde in experimental rats. BNNI.60(2):1-31.

Aebi, H. 1984. Catalase in vitro. In: Methods in enzymology. Academic Press. 105: 121- 126.

Barker, S.B. and Summerson, W.H. 1941. The colorimetric determination of lactic acid in biological material. J. Biol. Chem.138: 535-554.

Bergmeyer, H.O and Bruns, E. 1965. In: Methods of enzymatic analysis. Elsevier.

Bowerman, S., Wereszczynski, J., and Luger, K. 2021. Archaeal chromatin ‘slinkies’ are inherently dynamic complexes with deflected DNA wrapping pathways. Elife. 10: e65587

Carlberg, I. and Mannervik, B. 1985. Glutathione reductase. Meth. Enzymol.113: 484- 485.

Carroll, N.V., Longley, R.W., and Roe, J.H. 1956. The determination of glycogen in liver and muscle by use of anthrone reagent. J. Biol. Chem.220: 583-593.

Deepak, C., Rani, K.J., Shyama, K. and Ally, K. 2020. Effect of dietary incorporation of Ksheerabala residue on growth performance in Wistar rats. J. Vet. Anim. Sci. 51: 179-183.

Dingler, F.A., Wang, M., Mu, A., Millington, C.L., Oberbeck, N., Watcham, S., Pontel, L.B., Kamimae-Lanning, A.N., Langevin, F., Nadler, C. and Cordell, R.L. 2020. Two aldehyde clearance systems are essential to prevent lethal formaldehyde accumulation in mice and humans. Mol. Cell. 80: 996-1012.

Ezekiel, K.O. 2021. Lipid peroxidation and the redox effects of polyherbal. Intech Open. doi: 10.5772/intechopen.97625

Forner, F., Foster, L.J., Campanaro, S., Valle, G. and Mann, M. 2006. Quantitative proteomic comparison of rat mitochondria from muscle, heart, and liver. Mol. Cell Proteomics. 5:608-19.

Friedemann, T.E. and Haugen, G.E. 1942. Pyruvic Acid: I. Collection of Blood for the Determination of Pyruvic and Lactic Acids. J. Biol. Chem.144: 67-77.

Huckabee, W.E. 1961. Abnormal resting blood lactate: II. Lactic acidosis. Am. J. Med. 30: 840-848.

Kamps, J.J., Hopkinson, R.J., Schofield, C.J. and Claridge, T.D. 2019. How formaldehyde reacts with amino acids. Commun. Chem. 2:1-4.

Kemp, A. and Van Heijningen, A. J. 1954. A colorimetric micro-method for the determination of glycogen in tissues. Biochem. J.56: 646.

Kerns, W.D., Pavkov, K.L., Donofrio, D.J., Gralla, E.J. and Swenberg, J.A. 1983. Carcinogenicity of formaldehyde in rats and mice after long-term inhALTion exposure. Cancer Res. 43: 4382-4392.

Klein, V.J., Irla, M., Gil López, M., Brautaset, T. and Fernandes Brito, L. 2022. Unravelling formaldehyde metabolism in bacteria: road towards synthetic methylotrophy. Microorganisms.10(2): 220.

Kocyigit, A. and Guler, E.M. 2021. Antioxidant/ pro-oxidant, anti-inflammatory, and Immunomodulatory Effects of Thymoquinone in Cancer Prevention and Treatment. In Handbook of Oxidative Stress in Cancer: Therapeutic Aspects. Springer Singapore. 1-14p.

Kotnis, M.S., Patel, P., Menon, S.N. and Sane, R.T. 2004. Renoprotective effect of Hemidesmus indicus, a herbal drug used in gentamicin-induced renal toxicity. Nephrology. 9:142-52.

Lohr, GW. and Waller, H.D. 1965. Glucose-6- phosphate Dehydrogenase:(Zwisch enferment). In Methods of enzymatic analysis. Academic Press. 744-751p.

Lowry, O., Rosebrough, N., Farr, A.L. and Randall, R. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem.193: 265-75.

Malek, F.A., Möritz, K.U. and Fanghänel, J. 2004. Effects of a single inhALTive exposure to formaldehyde on the open field behavior of mice. Int J Hyg Environ Health. 207:151-8.

Manan, M., Saleem, U., Ahmad, B., Aslam, N., Anwar, A. and Zafar, A. 2022. Anti-arthritic and toxicological evaluation of ethanolic extract of Alternanthera bettzickianain rats. Front. Pharmacol.13:1002037.

Mastanaiah, S., Chengal Raju, D. and Swami, K.S. 1978. Glucose-6-phosphate dehydrogenase activity in denervated amphibian gastrocnemius muscle. Curr. Sci.47: 874-876.

Misra, H.P. and Fridovich, I. 1972. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 247: 3170-5.

Nachlas, M.M., Margulies, S.I., Goldberg, J.D. and Seligman, A.M. 1960. The determination of lactic dehydrogenase with a tetrazolium salt. Anal. Biochem. 1: 317-26.

Neenu, O., Surej, J.B., Biju, C., Senthil Murugan, S., Muhammed, E. M., Arathi, S., Sreeja, S. J., Sabarinath, M., Amrutha, A. and Merin Sneha, C.T. 2022. Evaluation of in vitro degradability of total mixed ration by synergistic supplementation of herbal extract-probiotic mixture in ruminants. J. Vet. Anim. Sci. 53: 105-111.

Nielsen, G.D., Larsen, S.T. and Wolkoff, P. 2017. Re-evaluation of the WHO (2010) formaldehyde indoor air quality guideline for cancer risk assessment. Arch.Toxicol. 91: 35-61.

Ohkawa, H., Ohishi, N. and Yagi, K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351-8.

Oluwafemi, A.B., Olamide, O.T., Adefunke, A.O., Abraham, A.O., Ogunlana, O.O., Janet, O.O., Marraiki, N., Beshbishy, A.M., Welson, N. and Batiha, G.E. 2020. Ganoderma lucidum from red mushroom attenuates formaldehyde-induced liver damage in experimental male rat model. Biology. 9: 313.

Owen, B.A., Dudney, C.S., Tan, E.L. and Easterly, C.E. 1990. Formaldehyde in drinking water: comparative hazard evaluation and an approach to regulation. Regul. Toxicol. Pharmacol. 11: 220-36.

Paul, M., Sohag, M.S., Khan, A., Barman, R.K., Wahed, M.I., Khan, M.R. 2020. Pumpkin (Cucurbita maxima) seeds protect against formaldehyde-induced major organ damages. Heliyon. 6(8): 221471037

Payani, S., Mamatha, C., Chandraprakash, C. and Bhaskar, M. 2019. Protective role of (Bronco-T) against formaldehyde induced antioxidant, oxidative and histopathological changes in lung of male Wistar rats. Toxicol. Rep. 6:718-26.

Pietzke, M., Meiser, J. and Vazquez, A. 2020. Formate metabolism in health and disease. Mol. Metab. 33:23-37.

Pontel, L.B., Rosado, IV., Burgos-Barragan, G., Garaycoechea, J.I., Yu, R., Arends, M.J., Chandrasekaran, G., Broecker, V., Wei, W., Liu, L. and Swenberg, J.A. 2015. Endogenous formaldehyde is a hematopoietic stem cell genotoxin and metabolic carcinogen. Mol. Cell. 60:177- 88.

Prameelamma, Y. and Swami, K.S. 1975. Glutathione dehydrogenase activity in normal and denervated gastocnemius muscle of frog, Ranahexadactyla. Curr. Sci. 44: 739-740.

Ramachandraiahgari, R.M., Somesula, S.R., Adi, P.J., Mannur, I.S., Enamala, M. and Matcha, B. 2012. Protective role of ethanolic extract of Aloe vera antioxidant properties on liver and kidney of streptozotocin-induced diabetic rats. Dig. J. Nanomater. Biostructures. 7:175- 84.

Reitman, S. and Frankel, S. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28:56-63.

Sasi, S., Sathu, T., Sunanda, C., Pavan, M., Vasudevan, V.N., Irshad, A. and Kumar, S.K., 2020. Effect of jackfruit powder on the physicochemical and sensory attributes of poultry meat cocktail nuggets. J. Vet. Anim. Sci. 51: 108 – 114.

Sayyar, Z., Yazdinezhad, A., Hassan, M. and Jafari Anarkooli, I. 2018. Protective effect of Matricaria chamomilla ethanolic extract on hippocampal neuron damage in rats exposed to formaldehyde. Oxid. Med. Cell. Longev. 2018: 6414317

Schurr, P. E., Thompson, H.T., Henderson, L.M. and Elvehjem, C. A. 1950. A method for the determination of free amino acids in rat organs and tissues. J. Biol. Chem.182: 29-37.

Sholapuri, P., Chintha, V., Matcha, B. and Pradeepkiran, J. 2020. Beneficial effects of polyherbal formulation (Bronco-T) on formaldehyde-induced lung toxicity in male Wistar rats. Toxicol. Res. 9:798- 807.

Singh, D., Cho, W.C. and Upadhyay, G. 2016. Drug-induced liver toxicity and prevention by herbal antioxidants: an overview. Front. Physiol. 6:363.

Tesfaye, S., Hamba, N., Gerbi, A. and Negeri, Z. 2021. Occupational formaldehyde exposure linked to increased systemic health impairments and counteracting beneficial effects of selected antioxidants. Alex. J. Med. 57:157-67.

Ueda, T.A., Arakawa, M.A. and Kotake, Y.A. 1967. Changes of aspartate aminotransferase and alanine aminotransferase activity in vitamin B6 deficient rat. Nagoya J. Med. Sci. 30:253-8.

Yalcin, E., Cavusoglu, K., Cicek, F., Demirtas, G. and Tasli, B. 2015. Histopathological and biochemical changes in Swiss albino mice induced by formaldehyde: protective effect of green tea extract. Cytologia.80(4):467-73.

Yao, D., He, Q., Bai, S., Zhao, H., Yang, J., Cui, D., Yu, Y., Fei, X., Mei, Y., Cheng, Y. and Yan, S. 2021. Accumulation of formaldehyde causes motor deficits in an in vivo model of hindlimb unloading. Commun. Biol. 4: 1-3.

Zhang, Y., Liu, X., McHale, C., Li, R., Zhang, L., Wu, Y., Ye, X., Yang, X. and Ding, S. 2013. Bone marrow injury induced via oxidative stress in mice by inhALTion exposure to formaldehyde. PloS one. 8: e74974.

Cite this article

Sujatha, B., Sholapuri, P. and Bhaskar M. 2023. Protective role of Bronco-T against formaldehyde-induced biochemical disruptions in the liver and muscle of male albino rats. J. Vet. Anim. Sci. 55(2):291-303

DOI: https://doi.org/10.51966/jvas.2024.55.2.291-303

Views
6
Downloads
1
Citations