Volume: 55 Issue: 1
Year: 2024, Page: 57-64, Doi: https://doi.org/10.51966/jvas.2024.55.1.57-64
Received: Sept. 21, 2023 Accepted: Nov. 24, 2023 Published: March 31, 2024
The present study aimed to evaluate the antibiotic susceptibility of Enterococcus sp. isolated from a household dahi/thayir sample. Bile esculin agar was used for the selective isolation of Enterococcus sp. Based on biochemical characterization and 16SrRNA sequencing, the isolate was identified as Enterococcus faecium, the sequence of which is deposited in NCBI with accession number OR105180. Enterococcus faecium was found to be resistant to Penicillin, Ampicillin, Vancomycin, Tetracycline, and Erythromycin. Multiple Antibiotic Resistance (MAR) index of the isolate was found to be 0.625. Minimum inhibitory concentration for Penicillin, Ampicillin, Vancomycin, Tetracycline and Erythromycin exceeded the break point established by CLSI guidelines. Microtiter plate assay revealed the isolate to be a weak biofilm former. Safety assessment revealed the isolate to be alpha haemolytic with no gelatinase activity. The observations endorse the possibility of household dahi/thayir samples being a potential reservoir for the dissemination of antibiotic resistance to human gut microflora.
Keywords: Enterococcus faecium, antibiotic resistance, multiple antibiotic resistance (MAR) index
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© 2024 Aiswarya et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Aiswarya, S.R., Beena, A.K. Aparana, S.V., Archana, C. and Aysha, C.H. 2024. Antibiogram of Enterococcus sp. isolated from household thayir sample. J. Vet. Anim. Sci. 55(1):57-64
DOI: https://doi.org/10.51966/jvas.2024.55.1.57-64