Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Antimicrobial resistance profile of Salmonella Typhimurium isolated from meat samples of Marathwada region, Maharashtra, India

D.D. Chilkewar1, R.N. Waghamare1*, P.B. Ghorpade2, D.B. Rawool3, K.K. Khose4 and R.L. Rasve1

1. Department of Veterinary Public Health College of Veterinary and Animal Sciences, Parbhani Maharashtra Animal and Fishery Sciences University, Nagpur Maharashtra, India

2. Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Parbhani Maharashtra Animal and Fishery Sciences University, Nagpur Maharashtra, India

3. National Research Centre on Meat, Chengicherla, Hyderabad.

4. Department of Poultry Science, College of Veterinary and Animal Sciences, Parbhani Maharashtra Animal and Fishery Sciences University, Nagpur Maharashtra, India

*Corresponding author: [email protected], Ph. 9766940954

Year: 2024, Page: 14-24, Doi: https://doi.org/10.51966/jvas.2024.55.1.14-24

Received: Aug. 29, 2023 Accepted: Dec. 27, 2023 Published: March 31, 2024

Abstract

The current study was planned to assess the antimicrobial resistance profile of Salmonella spp. isolated from meat samples collected from the Marathwada region. A total of 180 meat samples comprising 60 each of chicken, chevon and meat products were collected from 3 districts of Marathwada. The isolation of Salmonella spp. was carried out as per IS-5887 (Part 3): 1999 protocol and confirmed by morphological characteristics, biochemical reactions and PCR (invA gene) assay. A total of 5 isolates with an occurrence rate of 2.78 per cent were recovered from the 180 samples analysed, with an occurrence rate of 0, 3.33 and 5 per cent, respectively, from chicken, chevon and meat products. All isolates were serotyped as Salmonella Typhimurium by identifying the spy gene by PCR assay. Antimicrobial susceptibility testing revealed higher resistance against Erythromycin (100%), Amoxy-clav (80%), Ceftazidime (80%), Ciprofloxacin (80%), Amoxicillin/ Sulbactam (60%), Chloramphenicol (40%) and Amikacin (40%), while sensitivity was recorded against Tetracycline (100%), Co-Trimoxazole (100%), Ampicillin/Sulbactam (80%) and Gentamicin (80%). The average multiple antibiotic resistance (MAR) index of Salmonella Typhimurium isolates was 0.372. Genotypic resistance pattern revealed that all isolates were carrying the blaTEM gene (100%), while none of the isolates carried tetA and Sul1 gene, which correlated with phenotypic AST results. The study concluded that the occurrence of multiple antibiotic-resistant Salmonella Typhimurium in meat could pose a high risk to human health; therefore, continuous monitoring of multidrug resistance Salmonella spp. in animal origin food supply chain is essential to control the outbreaks.

Keywords: Salmonella typhimurium, antimicrobial resistance, PCR assay, blaTEM gene and food supply chain.

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Cite this article

Chilkewar, D.D., Waghamare, R.N., Ghorpade, P.B., Rawool, D.B., Khose K.K., and Rasve R.L. 2024. Antimicrobial Resistance Profile of Salmonella Typhimurium isolated from meat samples of Marathwada region, Maharashtra, India. J. Vet. Anim. Sci. 55(1):14-24

DOI: https://doi.org/10.51966/jvas.2024.55.1.14-24

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