Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Antibiogram of Enterococcus sp. isolated from household thayir sample

S.R. Aiswarya1*, A.K. Beena1, S.V. Aparana1, C. Archana2 and C.H. Aysha1

1. Department of Dairy Microbiology Verghese Kurien Institute of Dairy and Food Technology, Mannuthy, Thrissur, Pin-680651

2. Department of Dairy Microbiology, CDST, KAVSU, Pookode

Kerala Veterinary and Animal Sciences University Kerala, India

*Corresponding author: [email protected], Ph.9497131103

Year: 2024, Page: 57-64, Doi: https://doi.org/10.51966/jvas.2024.55.1.57-64

Received: Sept. 21, 2023 Accepted: Nov. 24, 2023 Published: March 31, 2024

Abstract

The present study aimed to evaluate the antibiotic susceptibility of Enterococcus sp. isolated from a household dahi/thayir sample. Bile esculin agar was used for the selective isolation of Enterococcus sp. Based on biochemical characterization and 16SrRNA sequencing, the isolate was identified as Enterococcus faecium, the sequence of which is deposited in NCBI with accession number OR105180. Enterococcus faecium was found to be resistant to Penicillin, Ampicillin, Vancomycin, Tetracycline, and Erythromycin. Multiple Antibiotic Resistance (MAR) index of the isolate was found to be 0.625. Minimum inhibitory concentration for Penicillin, Ampicillin, Vancomycin, Tetracycline and Erythromycin exceeded the break point established by CLSI guidelines. Microtiter plate assay revealed the isolate to be a weak biofilm former. Safety assessment revealed the isolate to be alpha haemolytic with no gelatinase activity. The observations endorse the possibility of household dahi/thayir samples being a potential reservoir for the dissemination of antibiotic resistance to human gut microflora.

Keywords: Enterococcus faecium, antibiotic resistance, multiple antibiotic resistance (MAR) index

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Cite this article

Aiswarya, S.R., Beena, A.K. Aparana, S.V., Archana, C. and Aysha, C.H. 2024. Antibiogram of Enterococcus sp. isolated from household thayir sample. J. Vet. Anim. Sci. 55(1):57-64

DOI: https://doi.org/10.51966/jvas.2024.55.1.57-64

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