Volume: 55 Issue: 1
Year: 2024, Page: 14-24, Doi: https://doi.org/10.51966/jvas.2024.55.1.14-24
Received: Aug. 29, 2023 Accepted: Dec. 27, 2023 Published: March 31, 2024
The current study was planned to assess the antimicrobial resistance profile of Salmonella spp. isolated from meat samples collected from the Marathwada region. A total of 180 meat samples comprising 60 each of chicken, chevon and meat products were collected from 3 districts of Marathwada. The isolation of Salmonella spp. was carried out as per IS-5887 (Part 3): 1999 protocol and confirmed by morphological characteristics, biochemical reactions and PCR (invA gene) assay. A total of 5 isolates with an occurrence rate of 2.78 per cent were recovered from the 180 samples analysed, with an occurrence rate of 0, 3.33 and 5 per cent, respectively, from chicken, chevon and meat products. All isolates were serotyped as Salmonella Typhimurium by identifying the spy gene by PCR assay. Antimicrobial susceptibility testing revealed higher resistance against Erythromycin (100%), Amoxy-clav (80%), Ceftazidime (80%), Ciprofloxacin (80%), Amoxicillin/ Sulbactam (60%), Chloramphenicol (40%) and Amikacin (40%), while sensitivity was recorded against Tetracycline (100%), Co-Trimoxazole (100%), Ampicillin/Sulbactam (80%) and Gentamicin (80%). The average multiple antibiotic resistance (MAR) index of Salmonella Typhimurium isolates was 0.372. Genotypic resistance pattern revealed that all isolates were carrying the blaTEM gene (100%), while none of the isolates carried tetA and Sul1 gene, which correlated with phenotypic AST results. The study concluded that the occurrence of multiple antibiotic-resistant Salmonella Typhimurium in meat could pose a high risk to human health; therefore, continuous monitoring of multidrug resistance Salmonella spp. in animal origin food supply chain is essential to control the outbreaks.
Keywords: Salmonella typhimurium, antimicrobial resistance, PCR assay, blaTEM gene and food supply chain.
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© 2024 Chilkewar et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Chilkewar, D.D., Waghamare, R.N., Ghorpade, P.B., Rawool, D.B., Khose K.K., and Rasve R.L. 2024. Antimicrobial Resistance Profile of Salmonella Typhimurium isolated from meat samples of Marathwada region, Maharashtra, India. J. Vet. Anim. Sci. 55(1):14-24
DOI: https://doi.org/10.51966/jvas.2024.55.1.14-24