Volume: 55 Issue: 1
Year: 2024, Page: 202-205, Doi: https://doi.org/10.51966/jvas.2024.55.1.202-205
Received: Oct. 12, 2023 Accepted: Dec. 28, 2023 Published: March 31, 2024
Collagen membranes are one of the most commonly used tissue engineered scaffold. Collagen scaffolds could be effectively made by decellularising a variety of biological tissues like small intestinal submucosa, dermis, omentum, tunica vaginalis, etc. These scaffolds are effectively used for the reconstruction of hernial defects, oesophageal defects, urinary bladder wall substitution, as artificial periosteum, etc. Decellularisation can be done using different methods like physical, chemical, and enzymatic. The present study discusses the successful decellularisation of bovine tunica vaginalis by combining enzymatic and chemical methods using the Trypsin-EDTA protocol with Triton X-100 as a detergent and using it as a tissue engineered scaffold.
Keywords: Collagen scaffolds, decellularisation, bovine tunica vaginalis, Trypsin-EDTA protocol
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© 2024 Jishi et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Jishi, D.P., Anoop, S., Sudheesh, S.N., Vasudevan, V.N., John, M.K.D., Amal, P. and Syam, K.V. 2024. Decellularisation of tunica vaginalis for the production of tissue engineered scaffolds: A promising approach in tissue engineering. J. Vet. Anim. Sci. 55(1):202-205
DOI: https://doi.org/10.51966/jvas.2024.55.1.202-205