Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Short Communication

Decellularisation of tunica vaginalis for the production of tissue engineered scaffolds: A promising approach in tissue engineering

P. Jishi Das1, S. Anoop1, Sudheesh S. Nair2, V. N. Vasudevan3, K. D. John Martin4, Amal Prakash1 and Syam K. Venugopal1

1. Department of Veterinary Surgery and Radiology College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680651 Kerala Veterinary and Animal Sciences University Kerala, India

2. Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Pookode.

3. Department of Livestock Products Technology

4. University Veterinary Hospital, Kokkalai

∗Corresponding author: [email protected], Ph.9446792333

Year: 2024, Page: 202-205, Doi:

Received: Oct. 12, 2023 Accepted: Dec. 28, 2023 Published: March 31, 2024


Collagen membranes are one of the most commonly used tissue engineered scaffold. Collagen scaffolds could be effectively made by decellularising a variety of biological tissues like small intestinal submucosa, dermis, omentum, tunica vaginalis, etc. These scaffolds are effectively used for the reconstruction of hernial defects, oesophageal defects, urinary bladder wall substitution, as artificial periosteum, etc. Decellularisation can be done using different methods like physical, chemical, and enzymatic. The present study discusses the successful decellularisation of bovine tunica vaginalis by combining enzymatic and chemical methods using the Trypsin-EDTA protocol with Triton X-100 as a detergent and using it as a tissue engineered scaffold.

Keywords: Collagen scaffolds, decellularisation, bovine tunica vaginalis, Trypsin-EDTA protocol


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Cite this article

Jishi, D.P., Anoop, S., Sudheesh, S.N., Vasudevan, V.N., John, M.K.D., Amal, P. and Syam, K.V. 2024. Decellularisation of tunica vaginalis for the production of tissue engineered scaffolds: A promising approach in tissue engineering. J. Vet. Anim. Sci. 55(1):202-205