Volume: 55 Issue: 1
Year: 2024, Page: 226-228, Doi: https://doi.org/10.51966/jvas.2024.55.1.226-228
Received: Nov. 22, 2023 Accepted: Dec. 7, 2023 Published: March 31, 2024
The gyrA gene in Klebsiella spp. is a 441 base pair long gene coding for the DNA gyrase A subunit of the organism. The present study was undertaken for the isolation, identification, and molecular confirmation of Klebsiella spp. from the environmental, animal and human samples by targeting the gyrA gene. A total of 175 samples were collected from the human patients, water and soil of the patients’ domestic environment and faecal samples of the domestic animals reared in the patients’ households. All the samples were subjected to the isolation and identification of Klebsiella spp. by conventional cultural methods and biochemical characterisation. The current study identified the occurrence of Klebsiella spp. in 94.34, 20.00, 14.00 and 36.36 per cent of samples collected from human, soil, water and animal sources, respectively. The recovered isolates were subjected to PCR-based amplification of the gyrA gene and all the presumptively identified isolates were found to harbour the gyrA gene.
Keywords: Klebsiella spp., gyrA gene, PCR
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© 2024 Akhil et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Akhil, K., Asha, K., Vinod, V.K., Vergis, J., Sumod, K. and Deepthy, B.J.2024. Detection and molecular characterisation of Klebsiella spp in human, animal and environmental interface: A one health approach. J. Vet. Anim. Sci. 55(1):226-228
DOI: https://doi.org/10.51966/jvas.2024.55.1.226-228