Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Short Communication

Detection and molecular characterisation of Klebsiella spp in human, animal and environmental interface: A one health approach

K. Akhil1*, K. Asha1, V.K. Vinod1, J. Vergis1, K. Sumod2 and B. J. Deepthy3

1. Department of Veterinary Public Health College of Veterinary and Animal Sciences, Pookode, Wayanad-673576 Kerala Veterinary and Animal Sciences University Kerala, India

2. Department of Veterinary Microbiology, CVAS Pookode

3. Department of Microbiology, Dr. Moopens Medical College

*Corresponding author: [email protected], Ph. 9544949160

Year: 2024, Page: 226-228, Doi: https://doi.org/10.51966/jvas.2024.55.1.226-228

Received: Nov. 22, 2023 Accepted: Dec. 7, 2023 Published: March 31, 2024

Abstract

The gyrA gene in Klebsiella spp. is a 441 base pair long gene coding for the DNA gyrase A subunit of the organism. The present study was undertaken for the isolation, identification, and molecular confirmation of Klebsiella spp. from the environmental, animal and human samples by targeting the gyrA gene. A total of 175 samples were collected from the human patients, water and soil of the patients’ domestic environment and faecal samples of the domestic animals reared in the patients’ households. All the samples were subjected to the isolation and identification of Klebsiella spp. by conventional cultural methods and biochemical characterisation. The current study identified the occurrence of Klebsiella spp. in 94.34, 20.00, 14.00 and 36.36 per cent of samples collected from human, soil, water and animal sources, respectively. The recovered isolates were subjected to PCR-based amplification of the gyrA gene and all the presumptively identified isolates were found to harbour the gyrA gene.

Keywords: Klebsiella spp., gyrA gene, PCR

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Cite this article

Akhil, K., Asha, K., Vinod, V.K., Vergis, J., Sumod, K. and Deepthy, B.J.2024. Detection and molecular characterisation of Klebsiella spp in human, animal and environmental interface: A one health approach. J. Vet. Anim. Sci. 55(1):226-228

DOI: https://doi.org/10.51966/jvas.2024.55.1.226-228

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