Volume: 55 Issue: 3
Year: 2024, Page: 640-643, Doi: https://doi.org/10.51966/jvas.2024.55.3.640-643
Received: April 9, 2024 Accepted: May 2, 2024 Published: Sept. 30, 2024
In pigs, stress factors such as pathogenic infections, environmental conditions, and various managemental practices can lead to a disease condition known as Porcine respiratory disease complex (PRDC). The main viral agents associated with this condition are Porcine circovirus 2 (PCV2), Porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), porcine respiratory coronavirus (PRCV), and pseudorabies virus. A study was undertaken to assess whether PCV2 and PRRSV, alone or in combination, are present in cases of respiratory ailments of pigs in North Kerala. The presence of PCV2 was detected by polymerase chain reaction (PCR) targeting the open reading frame 2 (ORF2) of the virus and PRRSV was detected by reverse transcriptase PCR (RT-PCR) targeting ORF6. A total of 54 tissue and lung samples were collected and screened for the presence of PCV2 and PRRSV. Of the samples tested, PCV2 could be detected in 22 (40.74 %) and PRRSV could be detected in 13 (24.07 %) samples. Two tissue samples showed mixed infections with PCV2 and PRRSV. The result shows that viral agents associated with PRDC are prevalent in pigs in North Kerala and that coinfections are also present.
Keywords: Porcine respiratory disease complex, Porcine circovirus 2, Porcine reproductive and respiratory syndrome virus, polymerase chain reaction
Chae, C. 2016. Porcine respiratory disease complex: Interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, and Mycoplasma hyopneumoniae. Vet. J. 212: 1-6.
Chaithra, G., Ravishankar, C., Sebastian, S.R., Rajasekhar, R., Anoopraj, R., Mani, B.K., Sumod, K., Deorao, C.V., Logeshwaran, G., Nandhakumar, D. and John, K. 2020. Molecular characterisation of porcine reproductive and respiratory syndrome virus from pigs in Kerala. Virus disease. 31: 560-565.
Drolet, R., Larochelle, R., Morin, M., Delisle, B. and Magar, R. 2003. Detection rates of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and swine influenza virus in porcine proliferative and necrotizing pneumonia. Vet. Pathol. 40:143–148. doi: 10.1354/vp.40-2-143.
Ellis, J., Krakowka, S., Lairmore, M., Haines, D., Bratanich, A., Clark, E., Allan, G., Konoby, C., Hassard, L., Meehan, B., Martin, K., Harding, J., Kennedy, S. and McNeilly, F. 1999. Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. J. Vet. Diagn. Invest. 11: 3-14.
Fan, P., Wei, Y., Guo, L., Wu, H., Huang, L., Liu, J. and Liu, C. 2013. Synergistic effects of sequential infection with highly pathogenic porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Virol J. 10: 265.
Jessil J., Balakrishnan-Nair D.K., Anoopraj R., Priya P.M. and Chintu R. and Prasanna K.S. 2022. Porcine Dermatitis and Nephropathy Syndrome on natural infection of Porcine Circovirus Type-2 in Pigs in Kerala, Southern India. J. Vet. Anim. Sci. 54: 736-743.
Kashyap, P., Hiremath, J., Patil, S.S., Suresh, K.P., Chanda, M., Rahman, H. and Hemadri, D. 2016. Baseline survey of PRRSV in India. In VIROCON 2016 and International Conference on Global Perspectives in Virus Disease Management. IIHR Bengaluru, 7th-10th December, 2016.
Kumar, G.S., Sharma, R. and Paliwal, O.P. 2006. Occurrence and pathology of post weaning multisystemic wasting syndrome. In: Compendium of Invited Papers and Abstracts of XXIII Annual Conference of Indian Association of Veterinary Pathologists. p. 83.
Liu, J.K., Wei, C.H., Yang, X.Y., Dai, A.L. and Li, X.H. 2013. Multiplex PCR for the simultaneous detection of porcine reproductive and respiratory syndrome virus, classical swine fever virus, and porcine circovirus in pigs. Mol. Cell. Probes. 27: 149-152.
Niederwerder, M.C., Jaing, C.J., Thissen J.B. Cino-Ozuna A.G. McLoughlin K.S. and Rowland R.R. 2016. Microbiome associations in pigs with the best and worst clinical outcomes following co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). Vet. Microbiol. 188: 1–11.
Rajesh, J.B., Rajkhowa, S., Dimri, U., Prasad, H. and Pegu, S.R. 2019. Seroprevalence of PCV2 in north eastern hill states of India. Ind. J. Anim. Sci. 89: 119-122.
Rajkhowa, T.K., Jagan Mohanarao, G., Gogoi, A., Hauhnar, L. and Isaac, L. 2015. Porcine reproductive and respiratory syndrome virus (PRRSV) from the first outbreak of India shows close relationship with the highly pathogenic variant of China. Vet. Q. 35: 186-193.
Sinha, A., Shen, H.G., Schalk, S., Beach, N.M., Huang, Y.W., Meng, X.J., Halbur, P.G. and Opriessnig, T. 2011. Porcine reproductive and respiratory syndrome virus (PRRSV) influences infection dynamics of porcine circovirus type 2 (PCV2) subtypes PCV2a and PCV2b by prolonging PCV2 viremia and shedding. Vet. Microbiol. 152: 235–246. doi: 10.1016/j.vetmic.2011.05.005.
Somashekara, S., Ravishankar, C., Ravindran, R., Rajappan, A., Kanjirakkuzhiyil, S., Paravalappil Muraleedharan, A., Kanjully Vadukoottayil, M., Janardhan, A. and John, K. 2023. Molecular characterization and phylogenetic analysis of porcine circovirus 2 from Kerala, India. Virus disease. pp.1-8.
Vijayaragavan, S., Balakrishnan-Nair, D.K., Sajitha, I. S., Priya, P. M., Anoopraj, R., Devi, S.S., Ravishankar, C., Divya, C. and Saifudeen, S.M. 2021.Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs. J. Vet. Anim. Sci. 52: 250 – 256. DOI:https://doi.org/10.51966/jvas.2021.52.3.250-256.
Xu, X. G., Chen, G. D., Huang, Y., Ding, L., Li, Z. C., Chang, C. D., Wang, C. Y., Tong, D. W. and Liu, H. J. 2012. Development of multiplex PCR for simultaneous detection of six swine DNA and RNA viruses. J. Virol. Methods. 183: 69-74.
Yin, S.H., Xiao, C.T., Gerber, P.F., Beach, N.M., Meng, X.J., Halbur, P.G. and Opriessnig, T. 2013. Concurrent porcine circovirus type 2a (PCV2a) or PCV2b infection increases the rate of amino acid mutations of porcine reproductive and respiratory syndrome virus (PRRSV) during serial passages in pigs. Virus Res. 178: 445–451. doi: 10.1016/j.virusres.2013.09.007.
Zeng, Z., Liu, Z., Wang, W., Tang, D., Liang, H. and Liu, Z. 2014. Establishment and application of a multiplex PCR for rapid and simultaneous detection of six viruses in swine. J. Virol. Methods. 208: 102–106. doi: 10.1016/j.jviromet.2014.08.001.
Zhang, J., Wang, P., Xie, C., Ha, Z., Shi, N., Zhang, H., Li, Z., Han, J., Xie, Y., Qiu, X., Tao, Y., Jin, N. and Lu, H. 2022. Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs. Viruses. 14(2): 193. doi: 10.3390/v14020193. PMID: 35215787; PMCID: PMC8877551.
Zhu, H., Chang, X., Zhou, J., Wang, D., Zhou, J., Fan, B., Ni, Y., Yin, J., Lv, L., Zhao, Y. and He, K. 2021. Co‐infection analysis of bacterial and viral respiratory pathogens from clinically healthy swine in Eastern China. Vet. Med. Sci. 7: 1815-1819.
© 2024 Jayanth et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
: Jayanth, K.V., Chintu, R., Shashank, S., Rajasekhar, R., Ajith, J.G., Sumod, K., Madhanraj, N., Sri, R.L. and Koshy, J. 2023. Detection of Viral Agents Associated with Porcine Respiratory Disease Complex (PRDC) in pigs in North Kerala. J. Vet. Anim. Sci. 55 (3):640-643