Volume: 56 Issue: 1
Year: 2025, Page: 53-59, Doi: https://doi.org/10.51966/jvas.2025.56.1.53-59
Received: Sept. 24, 2024 Accepted: Dec. 7, 2024 Published: March 31, 2025
The experiment was conducted at Avian Research Station, Thiruvazhamkunnu, to study the effects of in ovo inoculation and dietary supplementation with Lactobacillus plantarum (L. plantarum) and mannan oligosaccharide (MOS) on broiler growth performance from day-old to 42 days. A total of 420 eggs were assigned to seven treatment groups, each with 60 eggs: T1 (0.2 mL normal saline, control), T2 (0.2 mL of 107 CFU L. plantarum), T3 (0.2 mL of 0.5 per cent MOS), T4 (combination of L. plantarum and MOS), T5 (dietary 107 CFU L. plantarum/kg feed), T6 (dietary 0.5 per cent MOS), and T7 (dietary combination of L. plantarum and MOS). Hatch weights were significantly higher in the in ovo inoculated groups. By six weeks, the dietary synbiotic group (T7) and in ovo synbiotic group (T4) had similar body weights, which was significantly (p<0.05) higher than all other groups. Cumulative body weight gain was higher in synbiotic groups (T4 and T7). The in ovo prebiotic group (T3) showed growth performance similar to the synbiotic groups throughout the study period. However, cumulative feed consumption and feed conversion ratio (FCR) were unaffected by the treatments. The study concludes that in ovo inoculation of prebiotic and synbiotic is as effective as their dietary supplementation for enhancing growth performance of broiler chicken.
Keywords: Lactobacillus plantarum, MOS, synbiotic, in ovo, growth performance
Abd-El-Latif, M.A. and Omar, M.O. 2023. Productive performance, digestibility, blood parameters and intestine microbiology of broiler chicks affected by prebiotic, probiotic and synbiotic addition. Egypt. Poult. Sci. J. 43: 217-237.
Abdel-Moneim, A.M.E., Elbaz, A.M., Khidr, R.E.S. and Badri, F.B. 2020. Effect of in ovo inoculation of Bifidobacterium spp. on growth performance, thyroid activity, ileum histomorphometry, and microbial enumeration of broilers. Probiotics Antimicrob. Proteins. 12: 873-882.
AOAC [Association of Official Analytical Chemists]. 2016. Official Methods of Analysis. (20th Ed.). Association of Official Analytical Chemists International, Rockville, Maryland, 1885p.
Barros, V.R.S.M.D., Lana, G.R.Q., Lana, S.R.V., Lana, Â.M.Q., Cunha, F.S.A. and Neto, J.V.E. 2015. β-mannanase and mannan oligosaccharides in broiler chicken feed. Ciênc. Rural. 45: 111-117.
Benites, V., Gilharry, R., Gernat, A.G. and Murillo, J.G. 2008. Effect of dietary mannan oligosaccharide from Bio-MOS or SAF-Mannan on live performance of broiler chickens. J. Appl. Poult. Res. 17: 471-475.
BIS [Bureau of Indian Standards]. 2007. Poultry Feed Specification. (5th Ed.). Bureau of Indian Standards, New Delhi, 30p.
Bogucka, J., Ribeiro, D.M., Bogusławska‐Tryk, M., Dankowiakowska, A., da Costa, R.P.R. and Bednarczyk, M. 2019. Microstructure of the small intestine in broiler chickens fed a diet with probiotic or synbiotic supplementation. J. Anim. Physiol. Anim. Nutr. 103: 1785-1791.
Bozkurt, M., Küçükyilmaz, K., Çatli, A.U. and Çinar, M. 2008. Growth performance and slaughter characteristics of broiler chickens fed with antibiotic, mannan oligosaccharide and dextran oligosaccharide supplemented diets. Int. J. Poult. Sci, 7: 969-977.
Das, R., Mishra, P. and Jha, R. 2021. In ovo feeding as a tool for improving performance and gut health of poultry: a review. Front. Vet. Sci. 8: 754246.
Duan, A.Y., Ju, A.Q., Zhang, Y.N., Qin, Y.J., Xue, L.G., Ma, X., Luan, W.M. and Yang, S.B. 2021. The effects of in ovo injection of synbiotics on the early growth performance and intestinal health of chicks. Front. Vet. Sci. 8: 658301. https://doi.org/10.3389/fvets.2021.658301
Dunislawska, A., Slawinska, A., Stadnicka, K., Bednarczyk, M., Gulewicz, P., Jozefiak, D. and Siwek, M. 2017. Synbiotics for broiler chickens-in vitro design and evaluation of the influence on host and selected microbiota populations following in ovo delivery. PloS one. 12: 168587. https://doi.org/10.1371/journal.pone.0168587
Elangia, N., Senthil Murugan, S., Chacko, B., Ananth, D. and Vergis, J. 2023. Nutrient digestibility and growth promoting potential of Lactobacillus plantarum culture as probiotics in broilers. J. Vet. Anim. Sci. 55: 330-337.
Ghasemi, H.A. and Taherpour, K. 2013. Comparative effects of probiotic, prebiotic and synbiotic supplements on performance, jejunal morphology, serum lipid profile and antibody response of broiler chicks. J. Livest. Sci. Technol. 1: 20-27.
Guo, W., Wang, C., Qin, K., Shi, H., Yang, X. and Yang, X. 2023. Lactobacillus plantarum injection at the embryonic stage alters the early growth performance and lipid metabolism of broilers by specific genera of bacteria. Poult. Sci. 102: 102522. https://doi.org/10.1016/j.psj.2023.102522.
Kadam, M.M., Barekatain, M.R., Bhanja, S.K., Iji, P.A. 2013. Prospects of in ovo feeding and nutrient supplementation for poultry: the science and commercial applications- a review. J. Sci. Food Agric. 93: 3654-3661.
Kamran, Z., Mirzaa, M.A., Ahmad, S., Samad, H.A., Sohail, M.U. and Saadullahb, M. 2013. Performance of broiler chickens fed mannan oligosaccharides as alternatives to antibiotics from one to twenty-two days of age. J. Anim. Plant Sci. 23: 1482-1485.
Karimian, R.A. and Rezaeipour, V. 2020. Effects of dietary Mannan-oligosaccharides and phytase supplementation alone or in combination on growth performance, serum metabolites, cecal microbiota activity and intestinal morphology in broiler chickens. Poult. Sci. J. 8: 27-32.
Lenkova, T., Nikonov, I., Kuznetsov, Y., Karpenko, L. and Balykina, A. 2019. Development of the probiotic feed supplement based on Lactobacillus plantarum to increase the broiler productivity. Int. J. Innov. Technol. Explor. Eng. 9: 2452-2454.
Liu, L., Li, L., Li, C., Wang, H., Zhang, X., Ren, Q., Zhang, H., Jin, N., Li, C. and Zhao, C. 2023. Effects of Lactiplantibacillus plantarum LPJZ-658 supplementation on the production, meat quality, intestinal morphology, and caecal microbiota of broilers chickens. Microorganisms. 11:1549.https://doi.org/10.3390/microorganisms11061549
Majidi-Mosleh, A., Sadeghi, A.A., Mousavi, S.N., Chamani, M. and Zarei, A. 2017. Effects of in ovo infusion of probiotic strains on performance parameters, jejunal bacterial population and mucin gene expression in broiler chicken. Braz. J. Poult. Sci. 19: 97-102.
McCann, M.E.E., Newell, E., Preston, C. and Forbes, K. 2006. The use of mannan-oligosaccharides and/or tannin in broiler diets. Int. J. Poult. Sci. 5: 873-879.
Panda, A.K., Shyam Sunder, G., Rama Rao, S.V. and Raju, M.V.L.N. 2006. Early nutrition enhances growth and speeds up gut development. Wld. Poult. 22: 15-16.
Pender, C.M., Kim, S., Potter, T.D., Ritzi, M.M., Young, M. and Dalloul, R.A. 2017. In ovo supplementation of probiotics and its effects on performance and immune-related gene expression in broiler chicks. Poult. Sci. 96: 1052-1062.
Peng, Q., Zeng, X.F., Zhu, J.L., Wang, S., Liu, X.T., Hou, C.L., Thacker, P.A. and Qiao, S.Y. 2016. Effects of dietary Lactobacillus plantarum B1 on growth performance, intestinal microbiota, and short chain fatty acid profiles in broiler chickens. Poult. Sci. 95: 893-900.
Reshma, R., Chacko, B., Anitha, P., Prasoon, S. and Chandrasekhar, L. 2023. Effect of probiotics and rice on ileal microbial count and intestinal morphometry in broiler chicken. J. Vet. Anim. Sci. 54: 443-449
Shah, R., Paswan, V., Alolofi, A. and Yadav, S. 2019. Effect of mannan oligosaccharide prebiotic supplementation on growth and production performance of broiler chickens. Int. J. Livest. Res. 9: 85-91.
Slawinska, A., Zampiga, M., Sirri, F., Meluzzi, A., Bertocchi, M., Tavaniello, S. and Maiorano, G. 2020. Impact of galactooligosaccharides delivered in ovo on mitigating negative effects of heat stress on performance and welfare of broilers. Poult. Sci. 99: 407-415.
Sohail, M.U., Ijaz, A., Younus, M., Shabbir, M.Z., Kamran, Z., Ahmad, S., Anwar, H., Yousaf, M.S., Ashraf, K., Shahzad, A.H. and Rehman, H. 2013. Effect of supplementation of mannan oligosaccharide and probiotic on growth performance, relative weights of viscera, and population of selected intestinal bacteria in cyclic heat-stressed broilers. J. Appl. Poultry Res. 22: 485-491.
Song, D., Li, A., Wang, Y., Song, G., Cheng, J., Wang, L., Liu, K., Min, Y. and Wang, W. 2022. Effects of synbiotic on growth, digestibility, immune and antioxidant performance in broilers. Animal. 16: 100497. https://doi.org/ 10.1016/j.animal.2022.100497
Stasiak, K., Slawinska, A. and Bogucka, J. 2021. Effects of probiotics, prebiotics and synbiotics injected in ovo on the microstructure of the breast muscle in different chicken genotypes. Animals. 11: 2944. https://doi.org/10.3390/ani11102944
Sun, Y., Zhang, S., Li, H., Zhu, J., Liu, Z., Hu, X. and Yi, J. 2022. Assessments of probiotic potentials of Lactiplantibacillus plantarum strains isolated from Chinese traditional fermented food: phenotypic and genomic analysis. Front. Microbiol. 13: 895132. https://doi.org/10.3389/fmicb.2022.895132
Swapnil, S., Kanagaraju, P., Srinivasan, G., Kumanan, K. and Rathnapraba, S. 2021. Effect of in ovo feeding of probiotic, prebiotic and synbiotic to Broiler embryos on growth performance, Mucin-2 gene expression and gut colonization of microbiota. Indian J. Anim. Sci. 91: 51-57.
Uni, Z. and Ferket, P.R. 2003. Enhancement of development of oviparous species by in ovo feeding. US Patent No. 6592878.
Wang, S., Peng, Q., Jia, H.M., Zeng, X.F., Zhu, J.L., Hou, C.L., Liu, X.T., Yang, F.J. and Qiao, S.Y. 2017. Prevention of Escherichia coli infection in broiler chickens with Lactobacillus plantarum B1. Poult. Sci. 96: 2576-2586.
© 2025 Malini et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Malini, K.K., Prasoon, S., Binoj, C., Harikrishnan, S., Vimal, A.M. and Sunilkumar N.S. 2024. Effect of in ovo inoculation and dietary supplementation of Lactobacillus plantarum, mannan oligosaccharide and its combination on growth performance of broiler chicken. J. Vet. Anim. Sci. 56 (1):53-59