Volume: 55 Issue: 2
Year: 2024, Page: 425-431, Doi: https://doi.org/10.51966/jvas.2024.55.2.425-431
Received: Feb. 13, 2024 Accepted: March 20, 2024 Published: June 30, 2024
Binder of sperm (BSP) proteins represent a major class of proteins present in seminal plasma of ungulates. These proteins play a crucial role in various stages of fertilisation. The proteins have been extensively studied in purebred exotic cattle and many other species of ungulates. However, limited studies have been conducted with respect to BSP proteins in Vechur bull. This study was directed at isolation and characterisation of BSP proteins in Vechur bull seminal plasma. Seminal plasma was harvested from 36 ejaculates obtained from six healthy adult Vechur bulls. The mean protein content of seminal plasma was determined to be 84.10 ± 2.73 mg/mL. The proportion of BSP proteins recovered by gelatin affinity chromatography was 37.89 ± 0.83 per cent of the total protein. The SDS-page profiling of seminal plasma proteins revealed 20 protein bands in the 10- 180 kDa region. Three bands were observed, corresponding to the molecular weights of 14.3, 13.1, and 30 kDa, representing BSP1, BSP3, and BSP5, respectively. This study identified BSP proteins as the major seminal plasma protein of Vechur bull seminal plasma.
Keywords: Vechur, seminal plasma, binder of sperm proteins
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© 2024 Xavier et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Xavier, S., Harshan, H.M., Jayakumar, C., Chacko, L., Shynu, M. and Kurien, E. 2024. Isolation and characterisation of binder of sperm proteins in Vechur bull seminal plasma. J. Vet. Anim. Sci. 55(2):425-431
DOI: https://doi.org/10.51966/jvas.2024.55.2.425-431