Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Isolation and molecular characterisation of Avibacterium paragallinarum from Japanese quail in Kerala

S. K. Amritha1*, R. Ambily1, P. M. Priya1, K. M. Binu1, Arun George2 and S. Surya1

1. Department of Veterinary Microbiology

2. Department of Veterinary Clinical Medicine, Ethics and Jurisprudence

College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University Kerala, India

*Corresponding author: [email protected], Ph. 7306791396

Year: 2024, Page: 142-148, Doi: https://doi.org/10.51966/jvas.2024.55.1.142-148

Received: Oct. 12, 2023 Accepted: Nov. 28, 2023 Published: March 31, 2024

Abstract

Infectious coryza (IC) is an upper respiratory tract disease of poultry, caused by Avibacterium paragallinarum (A. paragallinarum). The disease is of much economic importance due to increased culling rate and marked reduction in egg production. There are no published reports of IC in quail in Kerala. The present study involves isolation and identification of A. paragallinarum from quails with respiratory signs, employing conventional and molecular methods. Swabs from infra-orbital sinus and trachea were collected under sterile precautions from 16 quails brought for disease investigation to Departments of Veterinary Microbiology and Pathology, College of Veterinary and Animal Sciences, Mannuthy, during August 2022 to March 2023. Avibacterium paragallinarum was isolated from one sample in chocolate agar, Haemophilus test medium and blood agar as tiny dewdrop colonies. The isolate was further characterised by biochemical tests and confirmed by PCR targeting HPG-2 gene. Three samples were found positive for both HPG-2 gene and hagA gene. The representative amplicons were sequenced, analysed and compared with sequences in GenBank. Phylogenetic analysis based on hagA gene revealed that the sample clustered together with isolates from different parts of the world.

Keywords: Avibacterium paragallinarum, quail, HPG-2 gene, hagA gene

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Cite this article

Amritha S.K., Ambily R., Priya P.M., Binu K.M., George A. and Surya S. 2024. Isolation and molecular characterisation of Avibacterium paragallinarum from Japanese quail in Kerala. J. Vet. Anim. Sci. 55(1):142-148

DOI: https://doi.org/10.51966/jvas.2024.55.1.142-148

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