Volume: 55 Issue: 1
Year: 2024, Page: 53-56, Doi: https://doi.org/10.51966/jvas.2024.55.1.53-56
Received: Sept. 21, 2023 Accepted: Dec. 16, 2023 Published: March 31, 2024
Metagenomic DNA extraction and subsequent bioinformatic analysis is an easy method to analyse the divergence and relative abundance of microflora present in environment samples. Extraction of DNA from faeces of elephant is challenging due the presence of inhibitory substances in dung. DNA was extracted from faeces using cTAB lysis method and commercial kits. The V3-V4 region of 16S rRNA was amplified and was further used for library preparation using NEBNext® Ultra™ DNA Library Prep Kit for Illumina®India. Taxonomic analysis was done using V search against SILVA data base. Metagenomic analysis revealed that Bacteriodata, Verrucomicrobiota, Firmicutes, Spirochaetota, Fibrobacterota, Synergistota, Proteobacteria were the major phyla seen in elephant dung samples.
Keywords: Extraction of DNA, dung samples, elephant
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© 2024 Vaishnav et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Vaishnav, B., Ajith, K.S., Jasmine Rani, K. and Aravindakshan, T.V. 2024. Metagenomic profiling of elephant dung using V3 – V4 amplicon sequencing of 16 S rRNA. J. Vet. Anim. Sci. 55(1):53-56
DOI: https://doi.org/10.51966/jvas.2024.55.1.53-56