Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Metagenomic profiling of elephant dung using V3 – V4 amplicon sequencing of 16S rRNA

Vyshnav B1, K. S. Ajith1*, K. Ally1, K. Jasmine Rani1 and T. V Aravindakshan2

1. Department of Animal Nutrition College of Veterinary and Animal Sciences, Mannuthy, Thrissur - 680 651

2. Dept. of Animal Genetics and Breeding, CVAS, Mannuthy

Kerala Veterinary and Animal Sciences University Kerala, India

*Corresponding author: [email protected], Ph. 9447203151

Year: 2024, Page: 53-56, Doi: https://doi.org/10.51966/jvas.2024.55.1.53-56

Received: Sept. 21, 2023 Accepted: Dec. 16, 2023 Published: March 31, 2024

Abstract

Metagenomic DNA extraction and subsequent bioinformatic analysis is an easy method to analyse the divergence and relative abundance of microflora present in environment samples. Extraction of DNA from faeces of elephant is challenging due the presence of inhibitory substances in dung. DNA was extracted from faeces using cTAB lysis method and commercial kits. The V3-V4 region of 16S rRNA was amplified and was further used for library preparation using NEBNext® Ultra™ DNA Library Prep Kit for Illumina®India. Taxonomic analysis was done using V search against SILVA data base. Metagenomic analysis revealed that Bacteriodata, Verrucomicrobiota, Firmicutes, Spirochaetota, Fibrobacterota, Synergistota, Proteobacteria were the major phyla seen in elephant dung samples.

Keywords: Extraction of DNA, dung samples, elephant

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Cite this article

Vaishnav, B., Ajith, K.S., Jasmine Rani, K. and Aravindakshan, T.V. 2024. Metagenomic profiling of elephant dung using V3 – V4 amplicon sequencing of 16 S rRNA. J. Vet. Anim. Sci. 55(1):53-56

DOI: https://doi.org/10.51966/jvas.2024.55.1.53-56

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