Volume: 56 Issue: 1
Year: 2025, Page: 13-19, Doi: https://doi.org/10.51966/jvas.2025.56.1.13-19
Received: Aug. 21, 2024 Accepted: Sept. 20, 2024 Published: March 31, 2025
The study was designed to evaluate a modified syringe technique to prepare platelet rich plasma (PRP) from rat blood. Blood was collected by terminal cardiac puncture from adult male Wistar rats. It was subjected to double centrifugation for the separation of platelets. The PRP thus prepared was stored at 4 °C. The platelet recovery parameters such as platelet enrichment factor and platelet recovery rate were calculated for the quantitative assessment of PRP. Platelet count, mean platelet volume and platelet distribution width were obtained for assessment of platelets during storage. Microbiological, cytological and ultrastructural evaluations were done. The platelet recovery parameters suggested that the PRP obtained, satisfied the criteria to be used clinically and was of reasonable quality. Indicators of platelet activation suggested that there was no inadvertent activation of platelets during storage. The cytological and ultrastructural evaluation also suggested the same. It was concluded that PRP prepared from rat blood using modified syringe technique and stored at 4 °C could safely and effectively be used for daily homologous application for up to one week that depended on in situ activation of platelets.
Keywords: Platelet rich plasma, rat, modified syringe technique, assessment, storage
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© 2025 Reji et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Reji, V., Anoop, S., Syam, K.V., Sooryadas, S., Vasudevan, V.N., Varuna, P.P., Sudheesh, S.N., Deny, J. and John Martin, K.D. 2025. Modified syringe technique to prepare platelet rich plasma from rat blood, its quantitative assessment, and evaluation of platelets stored at 4 °C to determine its suitability for homologous application.
J. Vet. Anim. Sci. 56 (1):13-19