Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Molecular analysis of partial VP-2 gene amplified from rectal swab samples of diarrheic dogs in Kerala confirms the circulation of canine parvovirus genetic variant CPV-2c

L. Devi Krishna1, K.C. Bipin1, P.M. Deepa1, R.L. Rathish1, Renjith Sebastian2 and Muhasin Asaf3

1. Department of Veterinary Epidemiology and Preventive Medicine

2. Dept. of Veterinary Biochemistry

3. Dept. of Animal Genetics and Breeding

College of Veterinary and Animal Sciences, Pookode, Wayanad – 673 576 Kerala Veterinary and Animal Sciences University Kerala, India

*Corresponding author: [email protected], Ph. 9447153448

Year: 2024, Page: 178-183, Doi: https://doi.org/10.51966/jvas.2024.55.1.178-183

Received: Oct. 13, 2023 Accepted: Dec. 29, 2023 Published: March 31, 2024

Abstract

Canine parvovirus enteritis is caused by canine parvovirus -2 (CPV-2), which is a highly contagious disease, characterized by vomiting, foul-smelling bloody diarrhoea and myocarditis in young dogs. In the present study, a total of 50 faecal samples were collected from dogs with the symptoms of haemorrhagic gastroenteritis from hospitals under Kerala Veterinary and Animal Sciences University to study the molecular epidemiology of the CPV-2. The samples were examined for parvovirus by using VP2 gene-specific PCR for parvovirus. Forty-one (82 per cent) dogs were positive for CPV-2 by PCR assay. DNA sequencing was done for five PCR-positive samples, out of which four were characterized as CPV-2c, indicating that CPV type 2c is currently circulating in India. The partial VP2 analysis showed that these CPVs are closely related to Asian-CPV-2c, with unique amino acids at positions 297A, 324I, 370R and 426E. The remaining one was characterised as the new CPV-2a. Incidence of CPV-2c is the first report in Kerala.

Keywords: Canine parvovirus (CPV), CPV-2c, new CPV-2a strain, sequence

Cite this article

Krishna, D.L., Bipin, K.C., Deepa, P.M., Rathish, R.L., Sebastian, R. and Asaf, M. 2024. Molecular analysis of partial VP-2 gene amplified from rectal swab samples of diarrheic dogs in Kerala confirms the circulation of canine parvovirus genetic variant CPV-2c. J. Vet. Anim. Sci. 55(1):178-183

DOI: https://doi.org/10.51966/jvas.2024.55.1.178-183

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