Volume: 56 Issue: 1
Year: 2025, Page: 86-92, Doi: https://doi.org/10.51966/jvas.2025.56.1.86-92
Received: Sept. 29, 2024 Accepted: Nov. 23, 2024 Published: March 31, 2025
Anaplasma spp. are obligate intracellular organisms responsible for significant tick-borne diseases affecting both human and animal health. Microscopic examination of blood smears (n=139) from symptomatic cattle in the present study revealed Anaplasma marginale in 15.82 per cent of the samples. Further, species specific PCR targeting msp4 gene of Anaplasma marginale yielded positive results in 86 samples (61.87 per cent). Haematological analysis of the infected animals revealed a significant reduction in total leucocyte count, granulocyte count, total erythrocyte count, haemoglobin, volume of packed red cells and a significant increase in mean corpuscular haemoglobin compared to the healthy animals. Biochemical analysis revealed a significant increase in total bilirubin in the infected group of animals. Phylogenetic analysis of the msp4 sequence of A. marginale revealed close relationship of the isolate A. marginale Mannuthy to the isolates from Turkey and Malaysia, though with moderate divergence indicating a common lineage with these isolates. Thus, this study records the molecular characterisation of A. marginale along with the haematobiochemical alterations in the infected cattle that would pave the way for adopting appropriate treatment regimen and control strategies.
Keywords: Anaplasma, cattle, msp4, haematobiochemical
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© 2025 Archana et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Archana, P.S., Priya, M.N., Bindu, L., Radhika, R., Syamala, K., Justin Davis, K., Safeer, M.S. and Anandhabhairavi P. 2024. Molecular characterisation of Anaplasma marginale and associated haematobiochemical alterations in naturally
affected bovines of central Kerala. J. Vet. Anim. Sci. 56 (1):86-92