Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Short Communication

Molecular detection of Fowl adenovirus from broiler chicken in Kerala

B.S. Ayana1, S. Sankar1*, P.M. Priya1, S.R. Nair1 and B.D. Krishna2

1. Department of Veterinary Microbiology

2. Department of Veterinary Pathology

College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University Kerala, India

*Corresponding author: [email protected], Ph. 9495242106

Year: 2024, Page: 217-221, Doi: https://doi.org/10.51966/jvas.2024.55.1.217-221

Received: Oct. 15, 2023 Accepted: Jan. 1, 2024 Published: March 31, 2024

Abstract

Fowl adenovirus affects birds, mainly chicken, duck, geese, turkey, and pheasant. Fowl adenovirus comprises of 5 species and 12 serotypes, which causes a multitude of disease syndromes. The most economically significant diseases caused by Fowl adenovirus include inclusion body hepatitis and hepatitis-hydropericardium syndrome. In the present study, a total of 56 post-mortem samples were collected from broiler birds brought to the Department of Veterinary Microbiology, Department of Veterinary Pathology and some organised farms in Kerala. Postmortem samples of liver, heart, lungs, kidney, bursa of Fabricius and spleen were collected for molecular detection. DNA was extracted from the pooled tissue samples using phenol chloroform method and subjected to polymerase chain reaction, employing primers targeting the hexon gene. Seven out of 56 samples turned out to be positive in PCR (12 per cent). The study revealed the increasing prevalence of Fowl adenovirus among the broiler population of Kerala.

Keywords: Fowl adenovirus, broiler, hexon, IBH-HPS

References

Asrani, R.K., Gupta, B.K., Sharma, S.K., Singh, S.P. and Katoch, R.C. 1997. Hydropericardium-hepatopathy syndrome in Asian poultry. Vet. Rec141: 271–273.

Asthana, M., Singh, V.K., Kumar, R. and Chandra, R. 2011. Isolation, cloning and In silico study of hexon gene of fowl adenovirus 4 (FAV4) isolates associated with Hydro pericardium syndrome in domestic fowl. J Proteomics Bioinform4: 190-195.

 Du, D., Zhang, P., Li, X., Tian, H., Cheng, Y., Sheng, D., Han, X., Shan, Y., Li, X., Yuan, Y. and Zhang, H. 2017. Cell-culture derived fowl adenovirus serotype 4 inactivated vaccine provides complete protection for virus infection on SPF chickens. Avian Dis. 28: 182-188.

Dutta, B., Deka, P., Gogoi, S.M., Sarmah, M., Bora, M.K. and Pathak, D.C. 2017. Pathology of inclusion body hepatitis hydropericardium syndrome (IBH-HPS) in broiler chicken. Int. J. Chem. Stud. 5: 458-461.

El-Shall, N. A., El-Hamid, H. S. A., Elkady, M. F., Ellakany, H. F., Elbestawy, A. R., Gado, A. R. 2022. Epidemiology, pathology, prevention, and control strategies of inclusion body hepatitis and hepatitis-hydropericardium syndrome in poultry: a comprehensive review. Front. Vet. Sci. 9: 963199.

Grgic, H., Philippe, C., Ojkic, D. and Nagy, É. 2006. Study of vertical transmission of fowl adenoviruses.Can. J. Vet. Res.70: 230-233.

Kim, J.N., Byun, S.H., Kim, M.J., Kim, J.J., Sung, H.W. and Mo, I.P. 2008. Outbreaks of hydropericardium syndrome and molecular characterization of Korean fowl adenoviral isolates. Avian Dis52: 526-530.

Morshed, R., Hosseini, H., Langeroudi, A.G., Fard, M.H.B. and Charkhkar, S. 2017. Fowl adenoviruses D and E cause inclusion body hepatitis outbreaks in broiler and broiler breeder pullet flocks. Avian Dis61: 205-210.

Niczyporuk, J.S. 2016. Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland. Arch. Virol. 161: 33-42.

Russell, W.C. 2009. Adenoviruses: update on structure and function. J. Gen. Virol.    90:1-20.

Steer, P.A., O'rourke, D., Ghorashi, S.A. and Noormohammadi, A.H. 2011. Application of high‐resolution melting curve analysis for typing of fowl adenoviruses in field cases of inclusion body hepatitis. Aust. Vet. J8: 184-192.

Toogood, C.I., Crompton, J. and Hay, R.T. 1992. Antipeptide antisera define neutralizing epitopes on the adenovirus hexon. J. Gen. Virol73: 1429-1435.

Winterfield, R.W., Fadly, A.M. and Gallina, A.M. 1973. Adenovirus infection and disease. Some characteristics of an isolate from chickens in Indiana. Avian Dis. 17: 334– 342.

Xia, J., Yao, K.C., Liu, Y.Y., You, G.J., Li, S.Y., Liu, P., Zhao, Q., Wen Rui Wu, Y.P., Huang, X.B., Cao, S.J. and Han, X.F. 2017. Isolation and molecular characterization of prevalent Fowl adenovirus strains in southwestern China during 2015–2016 for the development of a control strategy. Emerg. Microbes Infect. 6: 1-9.

Yang, K., Song, H., Shi, X., Ru, J., Tan, S., Teng, Z., Dong, H., Guo, H., Wei, F. and Sun, S. 2022. Preparation of a polysaccharide adjuvant and its application in the production of a foot-and-mouth disease virus-like particles vaccine. Biochem. Eng. J184: 108479-108489.

Cite this article

Ayana, B.S., Sankar, S., Priya, P.M., Nair, S.R. and Krishna, B.D. 2024. Molecular detection of Fowl adenovirus from broiler chicken in Kerala. J. Vet. Anim. Sci. 55(1):217-221

DOI: https://doi.org/10.51966/jvas.2024.55.1.217-221

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