Volume: 55 Issue: 1
Year: 2024, Page: 149-155, Doi: https://doi.org/10.51966/jvas.2024.55.1.149-155
Received: Oct. 12, 2023 Accepted: Nov. 28, 2023 Published: March 31, 2024
Canine mammary tumours (CMT) are the second most common cancer in dogs after skin tumours and are frequently found in older, intact female dogs. Epithelial- Mesenchymal Transition (EMT) is identified as a major driving force in tumour progression and metastasis. The current study was intended to assess the expression of N- cadherin to analyse the invasive and metastatic nature of CMT. A total of 25 CMT cases presented to the University Veterinary Hospitals at Mannuthy and Kokkalai during the period from June 2022 to June 2023 were considered for the present study. By immunohistochemistry (IHC) 48 per cent of cases showed EMT by expressing the cadherin switch. Lymph node metastasis was observed in 4 out of 25 cases during the study period. The expression of N-cadherin could be appreciated in almost all cases which showed lymph node metastasis except for a single CMT sample. The present study confirms the relationship between the expression of N-cadherin in CMT and the presence of regional metastasis and tumour grade. Therefore, the presence of N-cadherin protein in mammary carcinomas could be considered for prognostication.
Keywords: Canine mammary tumour, epithelial mesenchymal transition, N-cadherin, immunohistochemistry
Buendia, A.J., Peñafiel-Verdu, C., Navarro, J.A., Vilafranca, M. and Sanchez, J. 2014. N-cadherin expression in feline mammary tumors is associated with a reduced E-cadherin expression and the presence of regional metastasis. Vet. Pathol. 51: 755-758.
Cassali, G. D., Lavalle, G. E., Ferreira, E., Estrela-Lima, A., De Nardi, A. B., Ghever, C., Sobral, R. A., Amorim, R. L., Oliveira, L. O., Sueiro, F. A. R., Beserra, H. E. O., Bertagnolli, A. C., Gamba, C. O., Damasceno, K. A., Campos, C. B., Araujo, M. R., Campos, L. C., Monteiro, L. N., Nunes, F. C., Horta, R. S., Reis, D. C., Luvizotto, M. C. R., Magalhaes, G. M., Raposo, J. B., Ferreira, A. M. R., Tanaka, N. M., Grandi, F., Ubukata, R., Batschinski, K., Terra, E. M., Salvador, R. C. L., Jark, P. C., Juliana E. R., Delecrodi, J. E. R., Nascimento, N. A., Silva, D. N., Silva, L. P., Ferreira, K. C. R. S., Frehse, M. S., Santis, G. W., Silva, E. O., Guim, T. N., Kerr, B., Cintra, P., Silva, F., Leite, J., Mello, M., Ferreira, M. L. G., Fukumasu, H., Salgado, B. S. and Torres, R. 2014. Consensus for the diagnosis, prognosis and treatment of canine mammary tumors – 2013. Braz. J. Vet. Pathol. 7: 38-69.
Clemente, M., Perez-Alenza, M.D., Illera, J. C. and Pena, L. 2010. Histological, immunohistological, and ultrastructural description of vasculogenic mimicry in canine mammary cancer. Vet. Pathol. 47: 265-274.
Devi, S.S., George, A.J., Dhanushkrishna, B., Prasanna, K.S., Radhika, G. and Martin, K.J., 2022. Histomorphological stratification of stromal types associated with canine mammary tumours. J. Vet. Anim. Sci. 53: 688-693.
Di Domenico, M., Pierantoni, G.M., Feola, A., Esposito, F., Laino, L., De Rosa, A., Rullo, R., Mazzotta, M., Martano, M., Sanguedolce, F. and Perillo, L. 2011. Prognostic significance of N-Cadherin expression in oral squamous cell carcinoma. Anticancer Res. 31: 4211-4218.
Gill, K. S., 1997. Clinical studies on canine mammary tumours with reference to its surgical and chemotherapeutic management. M.V.Sc Thesis. Punjab Agricultural University, Ludhiana, 46p.
Goldschmidt, M., Peña, L., Rasotto, R. and Zappulli, V. 2011. Classification and grading of canine mammary tumors. Vet. Pathol. 48: 117-131.
Hatta, K. and Takeichi, M. 1986. Expression of N-cadherin adhesion molecules associated with early morphogenetic events in chick development. Nature. 320: 447-449.
Hazan, R.B., Qiao, R., Keren, R., Badano, I. and Suyama, K. 2004. Cadherin switch in tumour progression. Ann. N. Y. Acad. Sci. 1014: 155-163.
Hemanth, I., Kumar, R., Varshney, K. C., Nair, M. G., Kumar, B. R., Sivakumar, M. and Thanislass, J. 2015. Epidemiological and clinical studies on canine mammary tumors. I. J. Vet. Res. 24: 11-14.
Jechlinger, M., Grunert, S., Tamir, I.H., Janda, E., Lüdermann, S., Waerner, T., Seither, P., Weith, A., Beug, H. and Kraut, N. 2003. Expression Profiling of Epithelial Plasticity in Tumor Progression. Oncogene. 22: 7155-7169.
Mathew, R., Sajitha, I. S., Nair, S. S., Krishna, B. D. and Abraham, M. J. 2019. Canine mammary tumours: Histological malignancy grading as a prognostic indicator. Pharma. Innov. 8: 149-151.
Matos, A.J., Lopes, C. and Carvalheira, J. 2006. E-cadherin expression in canine malignant mammary tumors: relationship to other clinico-pathological variables. J. Comp. Pathol. 134: 182– 189.
Nakajima, S., Doi, R., Toyoda, E., Tsuji, S., Wada, M. and Koizumi, M. 2004. N-cadherin expression and epithelial-mesenchymal transition in pancreatic carcinoma. Clin. Cancer Res. 10: 4125–4133.
Nieman, M.T., Prudoff, R.S., Johnson, K.R. and Wheelock, M.J. 1999. N-cadherin promotes motility in human breast cancer cells regardless of their E-cadherin expression. J. Cell. Biol. 147: 631-644.
Patel, M. P., Ghodasara, D. J., Raval, S. H. and Joshi, B. P. 2019. Incidence, gross morphology, histopathology and immunohistochemistry of canine mammary tumours. IJVSBT. 14: 40-44.
Raval, S. H., Joshi, D. V., Parmar, R. S., Patel, B. J., Patel, J. G., Patel, V.B., Ghodasara, D. J., Chaudhary, P. S., Kalaria, V. A. and Charavada, A. H. 2018. Histopathological classification and immunohistochemical characterization of canine mammary tumours. Indian J. Vet. Pathol. 42: 19-27.
Rutteman, G.R. 1990. Hormones and mammary tumour disease in the female dog: an update. In Vivo (Athens, Greece). 4: 33-40.
Vakkala, M., Paakko, P. and Soini, Y. 1999. Expression of caspases 3, 6 and 8 is increased in parallel with apoptosis and histological aggressiveness of the breast lesion. Br. J. Cancer. 81: 592-599.
Wheelock, M. J., Soler, A. P. and Knudsen, K. A. 2001. Cadherin junctions in mammary tumours. J. Mammary Gland Biol. Neoplasia. 6: 275-285.
Wolfe, D. 2019. Tissue processing. In: Suvarna, K., Layton, C. and Bancroft, J.D. (ed.), Bancroft’s Theory and Practice of Histological Techniques. (8th Ed.). Elsevier Health Sciences, Churchill, 73pp.
© 2024 Anseena et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Anseena, A.K., Krithiga, K., Sajitha, I.S., Devi, S.S., Prasanna, K.S., Anoop, S. and Niyas, N. 2024. N-cadherin expression in canine mammary tumours. J. Vet. Anim. Sci. 55(1):149-155
DOI: https://doi.org/10.51966/jvas.2024.55.1.149-155