Volume: 56 Issue: 4
Year: 2025, Page: 600-605, Doi: https://doi.org/10.51966/jvas.2025.56.4.600-605
Received: Aug. 22, 2025 Accepted: Nov. 21, 2025 Published: Dec. 31, 2025
A total of 200 chicken intestines, which included (n=150) broilers and (n= 50) desi birds were collected from different locations of Puducherry. The faecal samples were examined both qualitatively and quantitatively for oocyst of Eimeria spp. Out of 200 intestinal samples, 60 samples were found positive for Eimeria spp. by qualitative examination. Following quantitative examination by McMaster Technique, the highest OPG (oocyst per gram) detected was 1,95,900 and the lowest OPG was 3800. The occurrence of Eimeria in chicken from Puducherry was 30 per cent. The overall Eimeria infection in broilers and desi birds was 30.66 per cent and 28 per cent, respectively. Examination of hemorrhagic and nodular lesions in GI tracts of both broilers and desi birds revealed the presence of oocysts of Eimeria either in caecal or intestinal forms. Histopathology studies revealed the presence of developing and developmental stages of Eimeria in the lamina propria, characterised by desquamation of enterocytes, as well as in the epithelial cells and submucosal cells, with massive infiltration of heterophils and mononuclear cells. Besides haemorrhages, degeneration and desquamation of cells, mild to moderate with lymphoid hyperplasia was observed.
Keywords: Occurrence, coccidiosis, qualitative examination, OPG, histopathology, Eimeriidae
Ashenafi, H. 2000. Survey on identification of major disease of chicken in three selected agro-climate zones in central Ethiopia: Unpublished DVM thesis, Faculty of Veterinary Medicine, Addis Ababa University, DebreZeit, Ethiopia.
Kumar, A., Tripathi, K.K., Niyogi, D., Singh, A., Gupta, R.K., Shrivastav, D.P., Alam, K. and Mishra, A. 2023. Incidence of coccidiosis in broiler birds in Ayodhya and Sultanpur district of Uttar Pradesh with special reference to study of Eimeria tenella. J. Exp. Zool. India. 26: 1987-1991.
Ahmed, A., Al-Gawad., Mahdy, O.A., Aida, A.N., El-Massry., Mohamed, S.A. and Al-Aziz, 2012. Studies on coccidia of Egyptian balady breed chickens. Lif. Sci. J. 9: 568-576.
Bachaya, H.A., Raza, M.A., Khan, M.N., Iqba, l.Z., Abbas, R.Z., Murtaza, S. and Badar, N. 2012. Predominance and detection of different Eimeria species causing coccidiosis in layer chickens. J. Anim. Plant. Sci. 22: 597-600.
Bachaya, H.A., Abbas, R.Z., Raza, M.A., Iqbal, Z., Rehman, T.U., Baber, W. and Hussain, R. 2014. Existence of coccidiosis and associated risk factors in broiler chickens in Southern Punjab, Pakistan. Pak. Vet. J. 35: 81-84.
Badawy, B.A., Tanios, N.I. and EL-Sawy, A.M. 2000. Effect of different stocking densities on severity and efficacy of treatment of Eimeria tenella in Balady chickens. J. Egypt. Vet. Med. Ass. 60: 19-30.
Blake, D.P., Knox, J., Deheck, B., Huntinton, B., Rathinam, T., Ravipati, V., Ayode, S., Gilbert, W., Adebambo, A.O., Jatau, I.D., Raman, M., Parker, D., Rushton, J. and Tomley, F.M. 2020. Re-Calculating the cost of coccidisosis in chicken. Vet. Res. 51: 115-130
Calnek, M. 1997. Diseases of Poultry, lowa State University Press, Ames.
Chapman, H.D. and Johnson, G.B. 1992. Oocyst of Eimeria in the litter of broilers reared to eight weeks of age. Poult. Sci. 7: 1342-1347.
Chauhan, H.V.S. and Roy, S. 2007. Poultry disease diagnosis and treatment, 3rd edition in India at Ramprinto graph Delhi.
Conway, D.P. and McKenzie, M.E. 1991. Poultry coccidiosis diagnostic and testing procedures. 2nd Edition, 2: 1736p.
Duszynski, D.W., Upton, S.J. and Couch, L. 1970. The coccidiosis of the world. Department of Biology, University of New Mexico Division of Biology, Kansas State University.
Elmusharaf, M.A. and Beyene, A.C. 2007. Department of nutrition, faculty of Veterinary Medicine, Utrecht university, the Netherlands.
Gar,i G., Tilaham, G. and Dorchies, P. 2008. Study on poultry coccidiosis in Tiyo District, Arsione, Ethiopia. Int. j. Poult. Sci. 7, 3: 251-256.
Guale, F. 1990. Poultry coccidiosis and effect of management System. An assessment trial in Debre Zeit and its surroundings. Faculty Veterinary Medicine, Addis Ababa University, Debre Zeit, DVM thesis.
Hamidinejat, H., Shapouri, M.R.S., Mayahi, M. and Borujeni, M.P. 2010. Characterization of Eimeria species in commercial broilers by PCR Based on ITS1 Regions of rDNA. Iranian. J. Parasitol. 5: 48-54.
Jadhav, B.N. and Nikam, S.V. 2014a. Study of seasonal incidence of chicken coccidiosis in Gangapur and Vaijapur Tehsil of Aurangabad District in Maharashtra. Int. J. Appl. Sci. Res. Rev. 1: 093-097.
Jatau, I.D., Sulaiman, N.H., Musa, I.W., Lawal, A.I., OkubanjoIsah, O.O. and Magaji, Y. 2012. Prevalence of coccidia infection and preponderance Eimeria species in free range indigenous and intensively managed exotic chickens during hot-wet season, in Zaria, Nigeria. Asian. J. Poult. Sci. 6: 79-88.
Jawad, T.I. and Jasim, G.A. 2025. Molecular detection and histopathological changes caused by Eimeria tenella in broilers in AlDiwaniyah Province, Iraq. Open. Vet. J. 15: 714-723.
Julie, D.H. 1999. Coccidiosis in poultry. Livest. Poult. Health. Program. 2: 3-4.
Kaboudi., Umar, S. and Munir, M.T. 2016. Prevalence of coccidiosis in free range chicken in Sidi, Thabet, Tunisia. Hindawi. Publis. Cor. Sci. 6.
Kers, J.G., Velkers, F.C., Fischer, E.A., Hermes, G.D.A., Stegeman, J.A. and Smiti, H. 2018. Host and Environmental factors affecting the intestinal microbiota in chickens. Front Microbial. 9:235.
Kim, W.H., Chaudhani, A.A. and Lillehoj, H.S. 2019. Involvement of T cell immunity in Avian coccidiosis. Front. Imm. 10: 2732.
Kumar, Y.R., Namratha, M.L., Sawale, G.K., Ramesh, G., Mahesh, B., Reddy, A.K. and Lakshman, M. 2019. Occurrence of intestinal and caecal coccidiosis in Rajasri birds. J. Anim. Res. 9: 875-878.
Kumar, A., Tripathi, K.K., Niyogi, D., Singh, A., Gupta, R.K., Shrivastav, D.P., Allan, K. and Mishra, A. 2023. Incidence of coccidisois in broiler bird in Ayodhya and Sultanpur district of Uttar Pradesh with special reference to study of Eimeria tenella. J. Exp. Zool. India. 26: 1987-1991.
Lawal, J.R., Jajere, S.M., Ibrahim, U.I., Geidem, Y.A., Gulani, I.A., Musa, G. and Ibekwe, B.U. 2016. Prevalence of coccidiosis among village and exotic bird of chickens in Maiduguri, Nigeria. Vet. World. 9: 653-659.
Luna, L.G. 1968. Manual of histological staining methods of the armed forces institute of pathology. 3rd Edn. L.G. Mcgnow-Hill Book Co, New York, USA.
Lunnden, A. and Thebo, P. 2000. Eimeria infection in litter-based, high stocking density systems for loose-housed laying hens in Sweden. Br. Poult. Sci. 41: 440-447.
Mc Dougald, L.R., Fuller, L. and Mattiello, R. 1997. A Survey of Coccidia on 43 Poultry Farms in Argentina. Avian. Dis. 41: 923-929.
McDougald, L.R. 2003. Coccidiosis. In diseases of poultry. 11th Ed. Iowa State Press, Blackwell Publishing Company, USA. 974-991.
Mokhtar, H. and Garedaghi, G. 2016. Prevalence of coccidiosis in broiler chicken farms in and around Marand city, Iran. J. Entomol. Zool. Stud. 4:174-177.
Naphade, S.T. 2013b. Studies on the incidence of infection of the disease coccidiosis in broiler chickens from in and around Aurangabad city. Ind. J. Sci. Res. Tech. 1: 39-43.
Nematollahi, A.G.H., Moghaddam R. and Pourabad F. 2009. Prevalence of Eimeria species among broiler chicks in Tabriz (Northwest of Iran). Mun. Ent. Zool. 4: 53-58.
Nghonjuyi, N.W., Kimbi, H.K. and Tiambo, C.K. 2014. Study of gastrointestinal parasites of scavenging chickens in Fiko division, Southwest Cameroon. J. Adv. Parasitol. 1: 30-34.
Oljira, D., Melaku, A. and Bogale, B. 2012. Prevalence and risk factors of coccidiosis in poultry farms in and around ambo town, Western Ethiopia. Am.-Eurasian J. Sci. Res. 7: 146-149.
Rasheda, M. and Bano, L. 1985. Histopathology of coccidiosis by Eimeria garnhami in Coturnix coturnix of N.W.F.P. Pak. Vet. J. 1: 27-29.
Ruzica, B., Neda, E., Cajavecl, S., Bara, V. and Tihomira, G. 2005. Immunoprophylaxis of coccidiosis-contribution to reducing of environment burden caused by intense poultry production. XIIth International Congress in Animal Hygiene, 4th to 8th September 2005, Warszaw, Poland, Warszaw Agricultural University, Vol. 2: 11-16.
Saravanajayam, M., Balasubramaniam, A. and Rani, N. 2016. Incidence of intestinal coccidiosis in desi chickens Tamil Nadu, India. J. Anim. Res. 6: 1061-1063.
Sharma, S., Iqbal, A., Azmi, S. and Shah, H.A. 2013. Study of poultry coccidiosis in organized and backyard farms of Jammu region. Vet. World. 6: 467–469.
Sharma, S., Iqbal, A., Azmi, S., Mushtaq, I., Wani, Z.A. and Ahmad, S. 2015. Prevalence of poultry coccidiosis in Jammu region of Jammu & Kashmir State. J. Parasit. Dis. 39: 85-89.
Stayer, P.A., Pote, L. and Mand, K. 1995. A comparison for Eimeria cysts isolated from litter and fecal samples from broiler house at two farm. Poult. Sci. 74: 26-32.
Sood, S., Yadav, A., Vohra, S., Katoch, R., Ahmad, B.D. and Borkatari, S. 2009. Prevalence of coccidiosis in poultry birds in R. S. Pura region, Jammu. Vet. Prac. 10: 69-70.
Soulsby, E.J.L. 1982. Helminths, Arthropods and Protozoa of Domesticated animals. 7th Ed. ELBS and Bailliere Tindall, London, 809p.
Tomza-Marciniak, A., Pilarczyk, B., Tobiańska, B. and Tarasewicz, N. 2014. Gastrointestinal parasites of free-range chickens. Ann. Parasitol. 60: 305-308.
Toulah, F.H. 2007. Prevalence and comparative morphological studies of four Eimeria spp. of sheep in Jeddah area, Saudi Arabia. J. Biol. Sci. 7: 413-416.
Witlock, D.R. and Ruff, M.D. 1977. Comparison of the intestinal surface damage caused by Eimeria mivati, E. necatrix, E. maxima, E.brunetti and E. acervulina by scanning electron microscopy. J. Parasitol. 63: 193 -199.
Yousuf, M., Tak, H. and Gul, N. 2013. Prevalence of Eimeriosis in domestic/ free range chickens of South Kashmir, (India). Int. J. Curr. Res. 5: 1606-1608.
© 2025 Shiyamala et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Shiyamala, S., Das, S.S., Sreekrishnan, R., Mathivathani, C. and Kumar, R. 2025. Occurrence of coccidiosis in chicken from Puducherry. J. Vet. Anim. Sci. 56 (4):600-605