Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Occurrence of diarrheagenic E. coli pathotypes in broiler chicken birds reared in Kerala

K. Asha1*, C. Sethulekshmi2, Renuka Nayar3, V. K. Vinod1, Jess Vergis1, K. Sumod4, V. Murugadas5 and Prejit1

1. Department of Veterinary Public Health College of Veterinary and Animal Sciences, Pookode- 673 576 Kerala Veterinary and Animal Sciences University Kerala, India

2. Dept of Veterinary Public Health, CVAS, Mannuthy

3. Dept of Livestock Products Technology, Pookode

4. Dept of Veterinary Microbiology, Pookode

5. MFB division, ICAR-CIFT, Kochi

*Corresponding author: [email protected], Ph. 9447235528

Year: 2024, Page: 39-46, Doi: https://doi.org/10.51966/jvas.2024.55.1.39-46

Received: Sept. 16, 2023 Accepted: Oct. 16, 2023 Published: March 31, 2024

Abstract

The diarrheal diseases caused by Escherichia coli (E. coli) are a very common occurrence in developing countries and the occurrence of pathogenic diarrheagenic E. coli (DEC) has been widely reported from different food animals from all over the world. The present study analysed 100 samples each of cloacal swabs and chicken slaughter waste from broiler chicken birds collected from two districts of Kerala. The samples were subjected to isolation and identification of E. coli using conventional method and biochemical characterisation and the isolates were confirmed using PCR amplification of uidA gene. The isolates were further subjected to detection of DEC pathotypes using PCR assay. The results of the analysis revealed that the overall occurrence of E. coli in broiler chicken samples from Wayanad and Ernakulam districts were 93.00 and 89.00 per cent, respectively. The most common DEC pathotype detected was EAEC followed by aEPEC. Only two isolates from Wayanad district carried genes suggestive of EHEC. None of the isolates from both the districts carried genes suggestive of ETEC.

Keywords: Diarrheagenic E. coli, broiler chicken, slaughter waste, virulence genes

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Cite this article

Asha, K., Sethulekshmi, C., Renuka N., Vinod, V.K., Vergis, J., Sumod, K., Murugadas, V. and Prejit. 2024. Occurrence of diarrheagenic E. coli pathotypes in broiler chicken birds reared in Kerala. J. Vet. Anim. Sci. 55(1):39-46

DOI: https://doi.org/10.51966/jvas.2024.55.1.39-46

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