Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Occurrence of diarrheagenic E. coli pathotypes in broiler chicken birds reared in Kerala

K. Asha1*, C. Sethulekshmi2, Renuka Nayar3, V. K. Vinod1, Jess Vergis1, K. Sumod4, V. Murugadas5 and Prejit1

1. Department of Veterinary Public Health College of Veterinary and Animal Sciences, Pookode- 673 576 Kerala Veterinary and Animal Sciences University Kerala, India

2. Dept of Veterinary Public Health, CVAS, Mannuthy

3. Dept of Livestock Products Technology, Pookode

4. Dept of Veterinary Microbiology, Pookode

5. MFB division, ICAR-CIFT, Kochi

*Corresponding author: [email protected], Ph. 9447235528

Year: 2024, Page: 39-46, Doi:

Received: Sept. 16, 2023 Accepted: Oct. 16, 2023 Published: March 31, 2024


The diarrheal diseases caused by Escherichia coli (E. coli) are a very common occurrence in developing countries and the occurrence of pathogenic diarrheagenic E. coli (DEC) has been widely reported from different food animals from all over the world. The present study analysed 100 samples each of cloacal swabs and chicken slaughter waste from broiler chicken birds collected from two districts of Kerala. The samples were subjected to isolation and identification of E. coli using conventional method and biochemical characterisation and the isolates were confirmed using PCR amplification of uidA gene. The isolates were further subjected to detection of DEC pathotypes using PCR assay. The results of the analysis revealed that the overall occurrence of E. coli in broiler chicken samples from Wayanad and Ernakulam districts were 93.00 and 89.00 per cent, respectively. The most common DEC pathotype detected was EAEC followed by aEPEC. Only two isolates from Wayanad district carried genes suggestive of EHEC. None of the isolates from both the districts carried genes suggestive of ETEC.

Keywords: Diarrheagenic E. coli, broiler chicken, slaughter waste, virulence genes


Afsal, S., Latha, C., Sethulekshmi, C., Binsy, M., Beena, C. J. and Gleeja, V. L. 2021. Occurrence of Escherichia coli in cloacal samples of broiler chicken from Kollam and Kottayam districts. J. Vet. Anim. Sci. 52: 371-376. jvas.2021.52.4.371-376.

Al Azad, M. A. R., Rahman, M. M., Amin, R., Begum, M. I. A., Fries, R., Husna, A., Khairalla, A. S., Badruzzaman, A. T. M., El Zowalaty, M. E., Lampang, K. N. and Ashour, H. M. 2019. Susceptibility and multidrug resistance patterns of Escherichia coli isolated from cloacal swabs of live broiler chickens in Bangladesh. Pathogens. 8: p118.

Alfifi, A., Christensen, J. P., Hounmanou, Y. M. G., Sandberg, M. and Dalsgaard, A. 2022. Characterization of Escherichia coli and other bacteria isolated from condemned broilers at a Danish abattoir. Front. Microbiol. 13: p1020586.

Amir, M., Riaz, M., Chang, Y. F., Ismail, A., Hameed, A. and Ahsin, M. 2021. Antibiotic resistance in diarrhoeagenic Escherichia coli isolated from broiler chickens in Pakistan. J. Food Qual. Hazards Control. 8: 78-86.

Bagheri, M., Ghanbarpour, R. and Alizade, H. 2014. Shiga toxin and beta-lactamases genes in Escherichia coli phylotypes isolated from carcasses of broiler chickens slaughtered in Iran. Int. J. Food Microbiol. 177: 16-20.

Barrow, G. I. and Feltham, R. K. A. 2003. Cowan and Steel’s Manual for Identification of Medical Bacteria. (3rd Ed) Cambridge press. 331p.

Bej, A. K., Dicesare, J. L., Haff, L. and Atlas, R. M. 1991. Detection of Escherichia coli and Shigella spp. in water by using the polymerase chain reaction and gene probes for uidAppl. Environ. Microbiol. 57: 1013-1017.

Bhave, S., Kolhe, R., Bhong, C., Jadhav, S., Nalband, S., Ranjan, M., Gandhale, D., Muglikar, D. and Deshpande, P. 2018. Isolation of diarrheagenic Escherichia coli from poultry faeces and raw chicken. J. Anim. Res. 8: 915-923.

Ekici, G. and Dümen, E. 2019. Escherichia coli and food safety. In The Universe of Escherichia coli. Starčič Erjavec, M. (Ed.) IntechOpen: London, UK. 17p.

Khalafalla, F. A., Abdel-Atty, N. S., Abdel-Wanis, S. A. and Hanafy, A. S. 2015. Food poisoning microorganisms in chicken broiler meat. Glob. Vet. 14: 211-218.

Mahmud, S., Nazir, K. N. H. and Rahman, M. T. 2018. Prevalence and molecular detection of fluoroquinolone-resistant genes (qnrA and qnrS) in Escherichia coli isolated from healthy broiler chickens. Vet. World. 11: 1720- 1724.

Mare, A. D., Ciurea, C. N., Man, A., Tudor, B., Moldovan, V., Decean, L. and Toma, F. 2021. Enteropathogenic Escherichia coli: A summary of the literature. Gastroenterol. Insights12: 28-40.

Muller, D., Greune, L., Heusipp, G., Karch, H., Fruth, A., Tschäpe, H. and Schmidt, M. A. 2007. Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR. Appl. Environ. Microbiol. 73: 3380-3390.

Nataro, J. P. and Kaper, J. B. 1998. Diarrheagenic Escherichia coliClin. Microbiol. Rev. 11: 142–201.

Valsalan, N., Alimudeen S., Vijin V. L., Francis, F. and Rajagopal, K. 2023. Consumption Pattern of Meat and Processed Meat Products in Kerala, India. Biol. Forum. 15: 71-74.

Vergis, J. 2013. Prevalence of diarrhoeagenic Escherichia coli pathotypes in human infants and young animals having diarrhoea (dissertation). Indian Veterinary Research Institute, Izatnagar, India. 99p.

Wang, L., Nakamura, H., Kage-Nakadai, E., Hara-Kudo, Y. and Nishikawa, Y. 2017. Prevalence, antimicrobial resistance and multiple-locus variable-number tandem-repeat analysis profiles of diarrheagenic Escherichia coli isolated from different retail foods. Int. J. Food Microbiol. 249: 44-52.

World Health Organization. 2015. WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015. World Health Organization. Geneva, Switzerland. Pp 265.

Cite this article

Asha, K., Sethulekshmi, C., Renuka N., Vinod, V.K., Vergis, J., Sumod, K., Murugadas, V. and Prejit. 2024. Occurrence of diarrheagenic E. coli pathotypes in broiler chicken birds reared in Kerala. J. Vet. Anim. Sci. 55(1):39-46