Volume: 55 Issue: 2
Year: 2024, Page: 317-324, Doi: https://doi.org/10.51966/jvas.2024.55.2.317-324
Received: Sept. 26, 2023 Accepted: April 16, 2024 Published: June 30, 2024
The present study was carried out with the objective to detect Listeria monocytogenes from raw milk and chicken meat. Two hundred samples of raw milk and chicken meat were obtained from local dairy farms and retail outlets, respectively in Thrissur, Kerala. These samples were enriched primarily in University of Vermont Medium-I (UVM I) broth, followed by further enrichment in UVM II broth. Subsequently, the enriched samples were streaked onto PALCAM agar for further analysis. Simultaneously, the samples were directly screened for presence of L. monocytogenes by PCR and real time PCR. Bacterial cultures that exhibited standard colony features were subsequently identified at the species level through the phenotypic tests. The isolates obtained were subjected to molecular confirmation, specifically targeting hlyA gene using conventional PCR and real time PCR. Additionally, the isolates were subjected to antibiotic sensitivity test. The overall occurrence of L. monocytogenes was 1.5 per cent, with two and one isolate being recovered from milk and meat, respectively. However, the occurrence was determined to be three per cent by PCR and real time PCR respectively. The identification of L. monocytogenes in raw milk and chicken meat highlights the importance to enforce adequate sanitation and hygiene measures in the food chain in order to protect the health of consumers.
Keywords: Occurrence, Listeria monocytogenes, hlyA, milk, meat
Anu S, 2012.Occurrence of Listeria species in beef, chicken and sea foods. M.V.Sc. thesis, Kerala Veterinary and Animal Sciences University, Pookode, 100p.
Aznar, R. and Alarcón, B., 2003. PCR detection of Listeria monocytogenes: a study of multiple factors affecting sensitivity. J. Appl. Microbiol.95: 958-966.
Barbuddhe, S.B., Chaudhari, S.P. and Malik, S.V.S. 2002.The occurrence of pathogenic Listeria monocytogenes and antibodies against listeriolysin-O in buffaloes.J. Vet. Med.49: 181-184.
Barbuddhe, S.B., Swain, B.K., Chakurkar, E.B. and Sundaram, R.N.S. 2003.Microbial quality of poultry meat with special reference to Listeria monocytogenes. Indian J. Poult. Sci.38: 305-307.
Bemrah, N., Sanaa, M., Cassin, M.H., Griffiths, M.W. and Cerf, O. 1998. Quantitative risk assessment of human listeriosis from consumption of soft cheese made from raw milk. Prev. Vet. Med.37: 129-145.
Bhilegaonkar, K.N., Kulshrestha, S.B., Kapoor, K.N., Kumar, A., Agarwal, R.K. and Singh, B.R. 1997. Isolation of Listeria monocytogenes from milk. J. Food Sci. Technol.34: 248-250.
Clinical and Laboratory Standards Institute (CLSI). 2017. Performance Standards for Antimicrobial Susceptibility Testing. Twenty Seventh Informational Supplement. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, USA. 250p.
Du, X.J., Zhang, X., Wang, X.Y., Su, Y.L., Li, P. and Wang, S. 2017. Isolation and characterization of Listeria monocytogenes in Chinese food obtained from the central area of China. Food Control.74: 9-16.
EFSA and ECDC (European Food Safety Authority and European Centre for Disease Prevention and Control), 2022. The European Union One Health 2021 Zoonoses Report, Italy. 273 p.
FAO and WHO. 2022. Listeria monocytogenes in ready-to-eat (RTE) foods: attribution, characterization and monitoring. Microbiological Risk Assessment Series No. 38, Rome. 202p
Griffiths, M.W. 1989. Listeria monocytogenes: its importance in the dairy industry. J. Sci. Food Agric.47: 133-158.
Hakenbeck, R. and Hof, H. 1991. Relatedness of penicillin-binding proteins from various Listeria species.FEMS Microbiol.Lett.84: 191-195.
Kalorey, D.R., Barbuddhe, S.B., Kurkure, N.V. and Gunjal, P.S. 2005.Prevalence of Listeria monocytogenes in poultry meat in Vidharba region of India.In XVIIth European Symposium on the Quality of Poultry Meat. 23rd May, 2005, Doorwerth. The Netherlands.
Latha, C., Anu, C.J., Ajaykumar, V.J. and Sunil, B. 2017.Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction.Vet. World.10: 927.
Menon, K.V., Sunil, B. and Latha, C., 2021. Prevalence and antibiotic resistance profile of Listeria spp. associated with seafoods from fish catchment areas in Kerala, India. Vet. World.14: 777.
Niederhauser, C., Candrian, U., Höfelein, C., Jermini, M., Bühler, H.P. and Lüthy, J., 1992.Use of polymerase chain reaction for detection of Listeria monocytogenes in food. Appl. Environ. Microbiol.58: 1564-1568.
Noll, M., Kleta, S. and Al Dahouk, S.2018. Antibiotic susceptibility of 259 Listeria monocytogenes strains isolated from food, food-processing plants and human samples in Germany. J. Infect. Public Health.11: 572-577.
Primer explorer 3.0, National Centre of Biotechnology Information (NCBI). https://www.ncbi.nlm.nih.gov/tools/ primer-blast/index.cgi, Accessed on May 15th 2022.
Rawool, D.B., Malik, S.V.S., Shakuntala, I., Sahare, A.M. and Barbuddhe, S.B., 2007. Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases. Int. J. Food Microbiol.113: 201-207.
Shakuntala, I., Das, S., Ghatak, S., Milton, A.A.P., Sanjukta, R., Puro, K.U., Pegu, R.K., Duarah, A., Barbuddhe, S.B. and Sen, A. 2019. Prevalence, characterization, and genetic diversity of Listeria monocytogenes isolated from foods of animal origin in North East India. Food Biotech.33: 237-250.
Shamloo, E., Hosseini, H., Moghadam, Z.A., Larsen, M.H., Haslberger, A. and Alebouyeh, M. 2019. Importance of Listeria monocytogenes in food safety: A review of its prevalence, detection, and antibiotic resistance. Iran. J. Vet. Res.20: 241.
Sharma, S., Sharma, V., Dahiya, D.K., Khan, A., Mathur, M. and Sharma, A., 2017. Prevalence, virulence potential, and antibiotic susceptibility profile of Listeria monocytogenes isolated from bovine raw milk samples obtained from Rajasthan, India. Foodborne Pathog. Dis.14: 132-140.
Soni, D.K., Singh, R.K., Singh, D.V. and Dubey, S.K. 2013. Characterization of Listeria monocytogenes isolated from Ganges water, human clinical and milk samples at Varanasi, India. Infect. Genet.Evol.14: 83-91.
Srinivasan, V., Nam, H.M., Nguyen, L.T., Tamilselvam, B., Murinda, S.E. and Oliver, S.P. 2005. Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathog. Dis. 2: 201-211.
USDA, 2018. United States Food and Drug Administration-Bacteriological Analytical Manual (U.S.FDA-BAM). 10: 35pp.
Vasu, R.K., Sunil, B., Latha, C., Menon, V. and Kumar, A. 2014. Prevalence of Listeria species in meat processing environments. Int. J. Curr. Microbiol. App. Sci. 3: 542-546.
Yamazaki, W., Taguchi, M., Kawai, T., Kawatsu, K., Sakata, J., Inoue, K. and Misawa, N. 2009. Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples. Appl. Environ. Microbiol.75: 1597-1603.
Youssef, M., Ramadan, H. and Al-Ashmawy, M. 2020. Prevalence of Listeria species in raw milk, ice cream and yogurt and effect of selected natural herbal extract on its survival. Mansoura Vet. Med. J.21: 99-106.
© 2024 Prasad et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Prasad, M.C.B., Menon, V.K., Milton, A.A.P., Sunil, B., Latha, C., Priya, P.M. and Thomas, N. 2024. Occurrence of Listeria monocytogenes in raw milk and chicken meat in Thrissur, Kerala. J. Vet. Anim. Sci. 55(2):317-324
DOI: https://doi.org/10.51966/jvas.2024.55.2.317-324