Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 3

  • Open Access
  • Research Article

Optimisation of induction and purification protocols of recombinant 22.6kDa tegumental protein of Schistosoma spindale inprokaryotic vector

Nikitha Shajan1, M.N Priya1*, Bindu Lakshmanan1, Amrutha Anand1, Ambily R.2, M.A Pradeep3 and Asha Rajagopal1

1 Department of Veterinary Parasitology, 2 Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651, Kerala Veterinary and Animal Sciences University, Kerala, India, 3 Central Marine Fisheries Research Institute, Kochi

Year: 2024, Page: 623-630, Doi: https://doi.org/10.51966/jvas.2024.55.3.623-630

Received: April 9, 2024 Accepted: May 16, 2024 Published: Sept. 30, 2024

Abstract

Schistosomosis is a prevalent zoonotic parasitic disease affecting both humans and animals on a global scale, with an estimated 165 million cattle and 200 million people impacted worldwide. Eventhough, serological methodologies designed for the identification of specific antibodies targeting parasitic antigens are esteemed for their high sensitivity, there are criticisms due to their incapacity to reliably indicate active infection, inability to correlate with the intensity of infection and lack of specificity. Enhancing the specificity of serological assays presents a significant challenge, primarily attributable to the identification and synthesis of specific antigens. In addressing these limitations, recombinant technology with specific immunogenic proteins as candidate antigens emerges as a viable alternative. In this study, induction of 22.6 kDa recombinant tegument protein of Schistosoma spindale was achieved using 0.6 mM concentration of IPTG at 37°C for four hours. Nickel chelating affinity chromatography was employed for protein purification, yielding maximum protein concentration at 75mM elution. Subsequently, the dialysis technique was employed to remove contaminants, while lyophilisation method was employed for protein concentration. The protein concentration postdialysis was measured at 0.220 mg/mL, while lyophilisation resulted in a concentration of 2mg/m

Keywords: 22.6 kDa tegumental protein, Schistosoma spindale, dialysis, lyophilisation

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Cite this article

: Nikitha, S., Priya, M.N., Bindu, L., Amrutha, A., Ambily, R., Pradeep, M.A. and Asha, R. 2024. Optimisation of induction and purification protocols of recombinant 22.6kDa tegumental protein of Schistosoma spindale in prokaryotic vector. J. Vet. Anim. Sci. 55 (3):623-630

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