Journal of Veterinary and Animal Sciences

Volume: 55 Issue: 1

  • Open Access
  • Research Article

Preparation and analysis of bovine platelet rich fibrin gel with special emphasis on histology and scanning electron microscopy

Karthik A. 1*, Laiju M. Philip1, John Martin K.D.2, Syam K. Venugopal1, Surej Joseph Bunglavan3 and Ajin S. Kumar1

1. Dept. of Veterinary Surgery and Radiology, College of Veterinary and Animal Science, Mannuthy, Thrissur - 680 651

2. University Veterinary Hospital, Kokkalai

3. University Livestock Farm and Fodder Research and Development Scheme, Mannuthy

Kerala Veterinary and Animal Sciences University Kerala, India.

*Corresponding author: [email protected], Ph. 7012497815

Year: 2024, Page: 88-93, Doi: https://doi.org/10.51966/jvas.2024.55.1.88-93

Received: Oct. 3, 2023 Accepted: Dec. 18, 2023 Published: March 31, 2024

Abstract

Platelet rich fibrin (PRF) has gained recognition as a regenerative biomaterial with potential applications in soft tissue healing. The study was aimed to prepare bovine origin platelet rich fibrin gel and characterisation by histology and scanning electron microscopy. PRF gel was prepared from venous blood collected from cross-bred postpartum dairy cattle centrifuged at 3200 rpm for 12 minutes. Histology and scanning electron microscopy revealed a compact fibrin network and the cells were distributed within PRF gel. The findings could provide a comprehensive understanding of cellular and matrix composition of bovine PRF gel, essential for its effective clinical application.

Keywords: Platelet rich fibrin gel, histological analysis, scanning electron microscopy, bovine PRF

References

Ardakani, M.R., Moein, H.P. and Beiraghdar, M. 2016. Tangibility of platelet-rich fibrin    matrix for nasolabial folds. Adv. Biomed. Res. 5. 197.

Bai, M.Y., Chuang, M.H., Lin, M.F., Tang, S.L., Wong, C.C. and Chan, W.P. 2018. Relationships of age and sex with cytokine content and distribution in human platelet fibrin gels. Sci. Rep. 8: 10642.

Bilgen, F., Ural, A. and Bekerecioglu, M. 2021. Platelet-rich fibrin: An effective chronic wound healing accelerator. J. Tissue Viability. 30: 616-620.

Charrier, J.B., Monteil, J.P., Albert, S., Collon, S., Bobin, S. and DM, D.E. 2008. Relevance of Choukroun's Platelet-Rich Fibrin (PRF) and SMAS flap in primary reconstruction after superficial or subtotal parotidectomy in patients with focal pleiomorphic adenoma: a new technique. Rev. Laryngol. Otol. Rhinol. 129: 313-318.

Choukroun, J., Diss, A., Simonpieri, A., Girard, M.O., Schoeffler, C., Dohan, S.L., Dohan, A.J., Mouhyi, J. and Dohan, D.M. 2006. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 101: 56-60.

Civinini, R., Macera, A., Nistri, L., Redl, B. and Innocenti, M. 2011. The use of autologous blood-derived growth factors in bone regeneration. Clin. Cases Miner. Bone Metab. 8: 25-31.

Demir, A., Erdikmen, D.O., Sevim, Z.T. and Altundağ, Y. 2022. The use of an autologous platelet-rich fibrin (PRF) membrane for the treatment of deep corneal ulcers in dogs. Vet. Arh. 92: 443-458.

Ehrenfest, D.D.M., de Peppo, G.M., Doglioli, P. and Sammartino, G. 2009. Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth factors27: 63-69.

Ehrenfest, D.D.M., Del Corso, M., Diss, A., Mouhyi, J. and Charrier, J.B. 2010. Three‐dimensional architecture and cell composition of a Choukroun's platelet‐rich fibrin clot and membrane. J. Periodontol. 81: 546-555.

 Ehrenfest, D.D.M., Pinto, N.R., Pereda, A., Jiménez, P., Corso, M.D., Kang, B.S., Nally, M., Lanata, N., Wang, H.L. and Quirynen, M. 2018. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte-and platelet-rich fibrin (L-PRF) clot and membrane. Platelets. 29: 171-184.

Feldman, A.T. and Wolfe, D. 2014. Tissue processing and hematoxylin and eosin staining. Histopathol. Methods Protoc. 31-43.

Gutierrez, D.A., Alfonso, C.A., Jaramillo-Isaza, S., Gomez, L.A. and Muñoz, A.L. 2021. Evaluation of platelet rich fibrin obtained using different centrifugation parameters as a tool for regenerative medicine. Clin. Lab. 67.

Jasmine, S., Thangavelu, A., Krishnamoorthy, R., Alzahrani, K.E. and Alshuniaber, M.A. 2021. Architectural and ultrastructural variations of human leukocyte-rich platelet-rich fibrin and injectable platelet-rich fibrin. J. Microsc. Ultrastruct. 9: 76.

Kang, Y.H., Jeon, S.H., Park, J.Y., Chung, J.H., Choung, Y.H., Choung, H.W., Kim, E.S. and Choung, P.H. 2011. Platelet-rich fibrin is a bioscaffold and reservoir of growth factors for tissue regeneration. Tissue Eng. Part A. 17: 349-359.

Raja, V.S. and Naidu, E.M. 2008. Platelet-rich fibrin: evolution of a second-generation platelet concentrates. Indian J. Dent. Res. 19: 42.

Roy, S., Driggs, J., Elgharably, H., Biswas, S., Findley, M., Khanna, S., Gnyawali, U., Bergdall, V.K. and Sen, C.K. 2011. Platelet‐rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation. Wound Repair Regen. 19: 753-766.

Sam, G., Vadakkekuttical, R.J. and Amol, N.V. 2015. In vitro evaluation of mechanical properties of platelet-rich fibrin membrane and scanning electron microscopic examination of its surface characteristics. J. Indian Soc. Periodontol. 19: 32-33.

Soares, C.S., Dias, I.R., Pires, M.A. and Carvalho, P.P. 2021. Canine-origin platelet-rich fibrin as an effective biomaterial for wound healing in domestic cats: A preliminary studyVet. Sci8: 213.

 Wang, Z., Han, L., Sun, T., Wang, W., Li, X. and Wu, B. 2019. Preparation and effect of lyophilized platelet-rich fibrin on the osteogenic potential of bone marrow mesenchymal stem cells in vitro and in vivo. Heliyon. 5: 10-15.

Wu, C.L., Lee, S.S., Tsai, C.H., Lu, K.H., Zhao, J.H. and Chang, Y.C. 2012. Platelet‐rich fibrin increases cell attachment, proliferation and collagen‐related protein expression of human osteoblasts. Aust. Dent. J. 57: 207-212.

Yang, A., Larsen, T.W. and Tume, R.K. 1992. Carotenoid and retinol concentrations in serum, adipose tissue and liver and carotenoid transport in sheep, goats and cattle. Aust. J. Agric. Res. 43: 1809-1817

Cite this article

Karthik A., Philip, L.M.., Martin, J.K.D., Venugopal, S.K.., Bunglavan, S.J.. and Kumar, A.S.. 2024. Preparation and analysis of bovine platelet rich fibrin gel with special emphasis on histology and scanning electron microscopy. J. Vet. Anim. Sci. 55(1):88-93

DOI: https://doi.org/10.51966/jvas.2024.55.1.88-93

Views
47
Downloads
22
Citations