Volume: 55 Issue: 2
Year: 2024, Page: 262-268, Doi: https://doi.org/10.51966/jvas.2024.55.2.262-268
Received: Dec. 30, 2022 Accepted: April 20, 2024 Published: June 30, 2024
The present study was envisaged to determine the prevalence of Brugia malayi in dogs in lymphatic filariosis endemic areas of Kerala. A total of 300 samples, consisting of 100 dog blood samples each from the endemic areas of Alappuzha, Ernakulam and Thrissur districts were screened. Out of 300 dogs screened by wet film technique, 56 (18.67 per cent) were positive for microfilariosis. Giemsa staining of blood smears revealed that 15 (five per cent) had sheathed microfilariae and 41 (13.67 per cent) had unsheathed microfilariae. All the samples found positive for sheathed microfilariae were subjected to histochemical differentiation for species identification. The occurrence of B. malayi in dog blood samples from Alappuzha, Ernakulam and Thrissur districts based on histochemical staining were one per cent, 1.33 per cent and 2.67 per cent, respectively. The overall occurrence of B. malayi was five per cent in the three districts. The present study confirmed the presence of B. malayi, a zoonotic pathogen, in dogs in Kerala.
Keywords: Brugia malayi, dog, histochemical staining
Ambily, V.R., Pillai, U.N., Arun, S., Pramod, S. and Jayakumar, K.M. 2011. Detection of human filarial parasite B. malayi in dogs by histochemical staining and molecular tecchniques. Vet. Parasitol. 181: 210- 214.
Chalifoux, L. and Hunt, R.D. 1971. Histochemical differentiation of Dirofilaria immitis and Dipetalonema reconditum. J. Am. Vet. Med. Assoc.158: 601-605.
Chirayath, D., Alex, P.C. and Pillai, U.N. 2017. Immunoblotting analysis of canine Brugia malayi microfilarial antigens.Trop. Biomed. 34: 815-821.
Kobasa, T., Thammapalo, S., Suvannadabba, S., Armesombun, A., Loymak, S., Leemingsawat, S. and Choochote, W. 2004. Identification of Brugia malayi like microfilariae in naturally infected cats from Narathiwat Province, Southern Thailand. J. Trop. Med. Parasitol. 27: 21- 25.
Loymek, S., Phuakrod, A., Zaelai, K., Sripumkhai, W., Vongjaroensanti, P. and Wongkamchai, S. 2021. Investigation on the prevalence of canine microfilaremia in Thailand using a novel microfluidic device in combination with real-time PCR. Vet. Sci. 8: 39p.
Mak, J.W., Yen, P.K., Lim, K.C. and Ramiah, N. 1980. Zoonotic implications of cats and dogs in filarial transmission in Peninsular Malaysia. Trop. Geogr. Med. 32: 259-64.
Mallawarachchi, C.H., Chandrasena, N.T., Premaratna, R., Mallawarachchi, S.M.N. and de Silva, N.R. 2018. Human infection with sub-periodic Brugia spp. In Gampaha District, Sri Lanka: a threat to filariosis elimination status?. Parasites Vectors. 11: 1-6.
Mathiarasan, L., Das, L. K. and Krishnakumari, A. 2021. Assessment of the impact of morbidity management and disability prevention for lymphatic filariosis on the disease burden in Villupuram district of Tamil Nadu, India. Indian J. Community Med.
Nuchprayoon, S., Junpee, A., Nithiuthai, S., Chungpivat, S., Suvannadabba, S. and Poovorawan, Y. 2006. Detection of filarial parasites in domestic cats by PCR-RFLP of ITS1. Vet. Parasitol. 140: 366-372.
Radhika, R. 1997. Prevalence, clinical pathology and treatment of microfilariosis in dogs in Thrissur. M.V.Sc thesis. Kerala Agricultural Universisty, Thrissur. 122p.
Rathnayake, S., Chandrasena, N., Wijerathna, T., Mallawarachchi, H. and Gunathilaka, N. 2022. Canine filaria species in selected lymphatic filariosis endemic and non-endemic areas in Sri Lanka. Parasitol. Res. pp. 1-5.
Ravindran, R., Varghese, S., Nair, S.N., Balan, V.M., Lakshmanan, B., Ashruf, R.M., Kumar, S.S., Gopalan, A.K., Nair, A.S., Malayil, A., Chandrasekhar, L., Juliet, S., Devada, K., Ramachandran, R., Regu, K. and Ali, S.M.K. 2014. Canine filarial infections in a human Brugia malayi endemic area of India. Bio. Med. Res. Int. 2: 1-9.
Rishniw, M., Barr, S. C., Simpson, K. W., Frongillo, M. F., Franz, M. and Alpizar, J. L. D. (2006). Discrimination between six species of canine microfilariae by a single polymerase chain reaction. Vet. Parasitol. 135: 303-314.
Sabu, L., Devada, K. and Subramanian, H. 2005. Dirofilariosis in dogs and humans in Kerala. Indian J. Med. Res.121: 691-693.
Sadarama, P. V., Chirayath, D., Pillai, U. N., Unny, M. and Lakshmanan, B. 2019. Preparation of excretory secretory protein from brugian microfilariae isolated from canine blood. J. Vet. Anim. Sci. 50: 23-25.
Tan, L. H., Fong, M. Y., Mahmud, R., Muslim, A., Lau, Y. L. and Kamarulzaman, A.2011. Zoonotic Brugia pahangi filariosis in a suburbia of Kuala Lumpur City, Malaysia. Parasitol. Int. 60: 111-11.
© 2024 Lakshmanan et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Lakshmanan, S. N., Latha, C. Sethulekshmi, C., Binsy, M., Bindu, L. and Aswathy, S. 2024. Prevalence of Brugia malayi in dogs in lymphatic filariosis endemic areas of Kerala. J. Vet. Anim. Sci. 55(2):262-268
DOI: https://doi.org/10.51966/jvas.2024.55.2.262-268