Volume: 55 Issue: 2
Year: 2024, Page: 402-412, Doi: https://doi.org/10.51966/jvas.2024.55.2.402-412
Received: Dec. 21, 2023 Accepted: Feb. 7, 2024 Published: June 30, 2024
The present study focuses on evaluating the probiotic potential and enzyme profiling of indigenous lactic acid bacteria. The cultures used were Lactiplantibacillus plantarum, two Lacticaseibacillus rhamnosus, Lactobacillus acidophilus, and Limosilactobacillus fermentum. In vitro analysis of acid and bile tolerance, cell surface hydrophobicity, aggregation potential, bile salt hydrolase activity, antimicrobial activities, and safety assessment were done for the isolates. All the cultures exhibited varying degrees of probiotic attributes confirming the strain specificity. The isolate Lactobacillus acidophilus, displayed the highest antimicrobial activity against both E. coli and S. aureus with zones of clearance measuring 16.50±0.50 mm and 19.00±1.00 mm, respectively. Enzyme profiling of the isolates was done for 19 enzymes and the results revealed the presence of four distinct enzymes in all cultures: leucine arylamidase, valine arylamidase, β-galactosidase, and β-glucosidase.
Keywords: Lactic acid bacteria, GRAS status, probiotics, API ZYM profile, enzymes
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© 2024 Sneha et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sneha, K., Aparna, S. V. and Beena, A. K. 2024. Probiotic potential assessment and enzyme profiling of indigenous Lactic Acid bacteria. J. Vet. Anim. Sci. 55(2):402-412
DOI: https://doi.org/10.51966/jvas.2024.55.2.402-412