Volume: 55 Issue: 3
Year: 2024, Page: 552-557, Doi: https://doi.org/10.51966/jvas.2024.55.3.552-557
Received: March 4, 2024 Accepted: April 17, 2024 Published: Sept. 30, 2024
The present study was conducted to standardise the protocol for microencapsulation of buck spermatozoa for its chilled preservation. Semen ejaculates collected from Malabari bucks of age two to three years, weighing 42 to 46 Kg and maintained under uniform managemental conditions were utilised. Sodium alginate of concentrations 0.5, 0.75 and 1.5 per cent with varying gauge sized hypodermic needles of 18G, 20G and 23G were used. The distance between the tip of the needle and the upper meniscus of BaCl2 solution was adjusted to varied distance like ≥ 7 cm, 3.5 to 5 cm, ≤ 2cm. This resulted in capsule sizes of 1 mm, 2 mm, 3 mm, 4 mm, 4.5 mm, 6 mm and 7 mm. Different shape of capsules like globular, plate like, irregular, tear-drop and oval were obtained and the capsule stability varied from 35 to 75 per cent. Capsule parameters were assessed at 0 h, 24 h, 48 h, 72 h and 96 h of chilled preservation in EYC extender at ratio of (1:2). The results of the present study cited that 1.5 per cent sodium alginate concentration, with 23G sized hypodermic needle and distance of 3.5 cm were ideal to acquire uniform spherical shaped stable capsule of size 1.90 ± 0.03 and stability of 75 per cent
Keywords: Microencapsulation, chilled preservation, buck spermatozoa, Malabari
Animal Husbandry Department. 2021. Report on Twentieth Quinquennial Livestock Census – 2019. Directorate of Animal Husbandry, Govt. of Kerala, Thiruvananthapuram, 203p.
Lakde, C., Patil, M., Sahatpure, S., Gawande, A. and Umesh, K. 2018. Standard protocol for encapsulation of canine semen. Int. J. Environ. Sci. 7: 1296-1300.
Max, X., Vacek, I. and Sun, A. 1994. Generation of alginate-poly-l-lysine-alginate (APA) biomicrocapsules: the relationship between the membrane strength and the reaction conditions. Artif. Cells Blood Substit. Immobil. Biotechnol. 22(1): 43-69.
Munkittrick, T.W., Nebel, R.L. and Saacke, R.G. 1992. Accessory sperm numbers for cattle inseminated with protamine sulfate microcapsules. J. Dairy Sci. 75: 25–31.
Nebel, R.L., Vishwanath, R., Mc Millan, W.H. and Saacke, R.G. 1993. Microencapsulation of bovine spermatozoa for use in artificial insemination: a review. Reprod. Fertil. Dev. 5: 701-712.
Nebel, R.L., Vishwanath, R., McMillan, W.H. and Pitt, C.J. 1996. Microencapsulation of bovine spermatozoa: effect of capsule membrane thickness on spermatozoal viability and fertility. Anim. Reprod. Sci. 44: 79-90.
Sachan, N.K., Pushkar, S., Jha, A. and Bhattcharya, A., 2009. Sodium alginate: the wonder polymer for controlled drug delivery. J. Pharm. Res. 2(8): 1191-1199.
Shah, S., Nagano, M., Yamashita, Y. and Hishinuma, M. 2010. Microencapsulation of canine sperm and its preservation at 4˚C. Theriogenology. 73: 560–567
Thiangthientham, P., Suwimonteerabutr, J., Tharasanit, T. and Techakumphu, M. 2020. The optimal divalent cations and storage temperatures for the encapsulation of ram spermatozoa. Thai J. Vet. Med. 50: 89–96.
: © 2024 Nivethitha et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Nivethitha K.K., Becha B.B., Jayakumar C., Bhai L.K. and Nair S.N. 2024. Standardisation of microencapsulation protocol for chilled preservation of Malabari buck spermatozoa. J. Vet. Anim. Sci. 55 (3):552-557