Volume: 55 Issue: 3
Year: 2024, Page: 612-615, Doi: https://doi.org/10.51966/jvas.2024.55.3.612-615
Received: March 12, 2024 Accepted: May 6, 2024 Published: Sept. 30, 2024
Substance P, a neurotransmitter in the central and peripheral nervous system, is considered as a biomarker of pain due to its key role in nociception. But recent researches have proved the increased expression of Substance P and its receptors in human breast cancers and their role in its progression. Canine mammary glands and spontaneously occurring neoplasms in them are considered best models for human breast cancers. Hence as a preliminary study, the present research work was undertaken to assess the level of Substance P in dogs with mammary tumours in comparison to healthy dogs. From the dogs brought for ovariohysterectomy and mammary tumour resection, 20 female dogs were selected for the study. Signalment and detailed history regarding each dog were recorded. All the dogs were subjected to thorough preanaesthetic evaluation and were grouped into Group I (mammary tumour) and II (control group) with 10 dogs each. Blood was collected from all the dogs and serum was separated and stored at -20ºC for estimation of substance P. The same was performed using sandwich ELISA technique with canine specific Substance P ELISA kit. The values obtained for Group I and II were statistically analysed using independent t- test. The level of substance P was significantly higher in the dogs of Group I when compared to Group II
Keywords: Substance P, mammary tumour, dogs
Al-Keilani, M. S., Elstaty, R. I., Alqudah, M. A. and Alkhateeb, A. M. 2021. Immunohistochemical expression of substance P in breast cancer and its association with prognostic parameters and Ki-67 index. PLoS One. 16: 1-16
Bille, C., Auvigne, V., Libermann, S., Bomassi, E., Durieux, P. and Rattez, E. 2012. Risk of anaesthetic mortality in dogs and cats: an observational cohort study of 3546 cases. Vet. Anaesth. Analg. 39: 59–68.
DeSantis, C. E., Ma, J., Gaudet, M. M., Newman, L. A., Miller, K. D., Goding Sauer, A. and Siegel, R. L. 2019. Breast cancer statistics, 2019. CA: Cancer J. Clin. 69: 438-451.
Ebrahimi, S., Javid, H., Alaei, A. and Hashemy, S. I. 2020. New insight into the role of substance P/neurokininâ1 receptor system in breast cancer progression and its crosstalk with microRNAs. Clin. Genet. 98: 322-330.
Esteban, F., Muñoz, M., González-Moles, M. A. and Rosso, M. 2006. A role for substance P in cancer promotion and progression: a mechanism to counteract intracellular death signals following oncogene activation or DNA damage. Cancer Metastasis Rev. 25: 137-145.
Ferreira, T., Gama, A., Seixas, F., Faustino-Rocha, A. I., Lopes, C., Gaspar, V. M. and Oliveira, P. A. 2023. Mammary Glands of Women, Female Dogs and Female Rats: Similarities and Differences to Be Considered in Breast Cancer Research. J. Vet. Sci. 10: 1-29.
Javid, H., Mohammadi, F., Zahiri, E. and Hashemy, S. I. 2019. The emerging role of substance P/neurokinin-1 receptor signaling pathways in growth and development of tumor cells. J. Physiol. Biochem. 75: 415-421.
O’Connor, T.M., O’Connell, J., O’Brien, D.I., Goode, T., Bredin, C.P. and Shanahan, F. 2004. The role of substance P in inflammatory disease. J. Cell. Physiol. 201: 167–180.
Robinson, P., Kasembeli, M., Bharadwaj, U., Engineer, N., Eckols, K. T. and Tweardy, D. J. 2016. Substance P receptor signaling mediates doxorubicin-induced cardiomyocyte apoptosis and triple-negative breast cancer chemoresistance. BioMed. Res. Int. 2016: 1-9
Rodriguez, E., Pei, G., Zhao, Z., Kim, S. T., German, A. and Robinson, P. 2021. Substance P antagonism as a novel therapeutic option to enhance efficacy of cisplatin in triple negative breast cancer and protect PC12 cells against cisplatin-induced oxidative stress and apoptosis. Cancers, 13: 1-15.
Yoon, J. S., Park, J., Song, R. and Yu, D. 2019. Substance P as a potential biomarker of pain assessment in dogs. Iran. J. Vet. Res. 20: 289-292.
© 2024 Ramankutty et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ramankutty, S., Anoop, S., Venugopal, S.K., Narayanan, M.K., Nisha, A.R. and Radhika, G. Substance P a potent prognostic indicator for canine mammary tumours- a comparative study. J. Vet. Anim. Sci. 55 (3):612-615